对《中国药典》2005年版蒲黄薄层鉴别、检查项修订的研究

目的：对《中国药典））2005年版一部中蒲黄薄层鉴别和检查项进行修订。方法：以香蒲新苷和异鼠李素-3-O-新橙皮苷作对照，采用聚酰胺薄膜，以丙酮-水（1：2）为展开剂进行鉴别；杂质检查时将筛分法与显微镜检相结合；采用2005年版药典附录Ⅸ K方法，测定了总灰分与酸不溶性灰分。结果：采用改进后的薄层鉴别方法对蒲黄中的香蒲新苷和异鼠李素-3-O-新橙皮苷进行鉴别，灵敏度高，斑点清晰，分离度好。采用筛分法进行杂质检查时，用7号药筛，称样量为10．0g，筛分时间为2min；增订的总灰分限度为10．0％，酸不溶性灰分的限度为4．0％。结论：改进后的薄层鉴别方法简便，快速，灵敏；修订后的检查项较为合理，更有利于蒲黄的质量控制。

Improvement on Identification and Impurity Test Methods for Pollen Typhae in ChP 2005

Objective： To improve the TLC identification and impurity test methods for Pollen Typhae in ChP 2005. Methods ： Polyamide film was chosen to be as coating substance, and acetone-water （1：2） was chosen to be as mobile phase to identify typhaeneoside and isorhamnetin-3-O-neohesperidoside. Sieving method combined with microscopy were used in impurity limit test. Total ash and acid insoluble ash were determined according to the method of Appendix IX K in Ch. P 2005. Results： Using the improved method, typhaeneoside and isorhamnetin- 3-O- neohesperidoside had more clear spots, higher sensitivity and better resolution. In impurity limit test, about 10. 0 g of the power was passed through No. 7 sieve for 2 rain to inspect foreign matter. The limits of total ash and insoluble ash were defined 10. 0% and 4. 0% , respectively. Conclusions ： The improved TLC identification method is simple, rapid, sensitive. The revised examination items are more reasonable. They are more useful for quality control of Pollen Typhae.