茶多酚及茶多酚锗的组成、结构和抑制肺癌细胞生长速率的研究

采用有机合成法合成茶多酚-锗化合物.运用反相液相色谱技术初步分离茶多酚和茶多酚锗的酚类组成.选用基质辅助激光解吸电离化飞行时间(MALDI-TOF)质谱技术分析茶多酚及茶多酚-锗的组成和结构.结果表明,茶多酚主要由儿茶素及酚类化合物组成,其中(-)表没食子儿茶素没食子酸酯(EGCG)、(-)表没食子儿茶素没食子棓酸甲酯(EGC-MG)和表三羟基黄烷-3-醇没食子酸酯(EZG)易与Ge4 反应且形成含锗的酚类化合物.茶多酚及茶多酚-锗化合物均有抑制肺癌细胞生长的能力,其抑制速率随着酚类化合物浓度增加而提高,但茶多酚锗抑制速率高于茶多酚近1倍.

Composition,Structure,and Inhibiting Growth Rate of Lung-cancer Cell by Tea Polyphenol and Tea Polyphenol-germanium

The compound of tea polyphenol-germanium(TP-Ge4 ) was synthesized by typical technology of organic synthesis.The polyphenol compositions of tea polyphenol and tea polyphenol-germanium were effectively separated by RE-HPLC techniques.Matrix-assisted laser desorption ionization/time of flight(MALDI-TOF) mass spectrometry was selected to analyze the composition,structure and stability of tea polyphenol(TP) and TP-Ge4 .The results showed that TP was mainly composed of catechin and polyphenolic compounds.These polyphenolic compounds such as epigallo-catechin gallate(EGCG),epigallo-catechinmethyl gallate(EGCMG),epiafzelechin gallate(EZG) reacted easily with germanium oxide to consistute a series compounds of the polyphenolic-germanium.In addition,both TP and TP-Ge4 showed capacities for inhibitting growth of lung-cancer cells.This inhibiting rate would enhance with the concentration increment both compounds.However,the inhibiting rate with TP-Ge4 showed higher approximately one multiple than that of TP.