| 靶向survivin的microRNA和siRNA抑制前列腺癌细胞survivin基因表达和凋亡作用的比较 |
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| 引用本文： | 骆晓梅,刘家云,苏明权,郝晓柯.靶向survivin的microRNA和siRNA抑制前列腺癌细胞survivin基因表达和凋亡作用的比较[J].第四军医大学学报,2009,30(4):331-335. |
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| 作者姓名： | 骆晓梅 刘家云 苏明权 郝晓柯 |
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| 作者单位： | 骆晓梅,LUO Xiao-Mei(解放军第97医院检验科,江苏徐州,221004);刘家云,苏明权,郝晓柯,LIU Jia-Yun,SU Ming-Quan,HAO Xiao-Ke(第四军医大学西京医院全军临床检验医学中心,陕西西安,710033)
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| 摘 要： | 目的：构建靶向survivin的microRNA表达载体，观察其与siRNA表达载体对survivin基因表达的抑制作用。方法：以survivin为靶基因，根据pPRIME设计针对survivin的microRNA序列，获得目的片段克隆到相应载体中，序列正确的载体脂质体转染前列腺癌PC-3细胞，RT-PCR和Western Blot检测其对survivin基因表达的抑制作用，流式细胞术和电镜观察其对细胞凋亡的影响。结果：双酶切鉴定、DNA测序证实构建的microRNA表达载体序列正确，序列正确的microR-NA和siRNA表达载体在mRNA和蛋白水平上均能明显抑制PC-3细胞中survivin的表达，流式细胞术和电镜结果显示其可促进PC-3细胞的凋亡，且microRNA表达载体的作用效果较siRNA表达载体强。结论：Survivin靶向microRNA和siRNA均能抑制靶基因的表达，诱导前列腺癌细胞凋亡，与siRNA相比，microRNA作用效果更强。
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| 关 键 词： | 靶向治疗 survivin 微RNAs RNA 小分子干扰 |
Comparison of inhibitory effects of microRNA and siRNA on survivin gene expression and apoptosis in prostate cancer |
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| LUO Xiao-Mei,LIU Jia-Yun,SU Ming-Quan,HAO Xiao-KePLA th Hospital,Xuzhou ,China,PLA Center for Clinical Laboratory Medicine,Xijing Hospital,Fourth Military Medical University,Xi''an.Comparison of inhibitory effects of microRNA and siRNA on survivin gene expression and apoptosis in prostate cancer[J].Journal of the Fourth Military Medical University,2009,30(4):331-335. |
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| Authors: | LUO Xiao-Mei LIU Jia-Yun SU Ming-Quan HAO Xiao-KePLA th Hospital Xuzhou China PLA Center for Clinical Laboratory Medicine Xijing Hospital Fourth Military Medical University Xi'an |
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| Affiliation: | LUO Xiao-Mei1,LIU Jia-Yun2,SU Ming-Quan2,HAO Xiao-Ke21PLA 97th Hospital,Xuzhou 221004,China,2PLA Center for Clinical Laboratory Medicine,Xijing Hospital,Fourth Military Medical University,Xi'an 710033 |
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| Abstract: | AIM:To design and construct microRNA expression vectors,and to compare microRNA and siRNA inhibitive effects on survivin gene expression.METHODS:Based on survivin gene sequence,targeted pre-microRNA oligonucleotides were designed and synthesized respectively,and cloned into established microRNA expression vector pPRIME.PC-3 cells were transiently transfected with correct constructs of microRNA and siRNA and the inhibitory effects were determined by PT-PCR and Western blot.The apoptosis index of PC-3 cells w... |
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| Keywords: | survivin |
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