低氧诱导的非酒精性脂肪性肝炎动物模型的构建

目的：构建低氧诱导的非酒精性脂肪性肝炎动物模型。方法50只大鼠分为正常组、低氧组、高脂组、低氧高脂组4组，分别给予正常饮食、腹腔内注射亚硝酸钠、高脂饮食、腹腔内注射亚硝酸钠＋高脂饮食。检测4组大鼠生化指标（ALT、AST、AKP、TG）、透明质酸（HA）、动脉高铁血红蛋白及氧分压，并行肝组织HE与Masson染色及NASH评分。结果低氧高脂组大鼠的ALT、AST、AKP、TG、HA明显升高，NASH评分明显增加，肝组织可见明显的脂肪变性、炎症细胞浸润及纤维化。结论高脂饮食大鼠诱导单纯脂肪肝基础上给予腹腔注射亚硝酸钠能够建立低氧诱导的非酒精性脂肪性肝炎动物模型。

Establishment of animal model for hypoxia-induced non-alcoholic steatohepatitis

Objective To establish a animal model for hypoxia- induced non- alcoholic steatohepatitis. Methods Rats were randomly assigned to a normal diet （control group）, normal diet＋intraperitoneal injection of sodium nitrite （hypoxia group）, high fat diet （high fat group）, intraperitoneal injection of sodium nitrite＋high fat diet （hypoxia＋high fat group）, respectively. Bio-chemical markers including alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, total triglycerides （TG） and liver fi-brosis marker hyaluronic acid （HA）, methemoglobin and arterial oxygen partial pressure were detected. In addition, liver tissue HE and Masson staining and NASH score were performed. Results The levels of ALT, AST, TG, HA and NASH score were signifi-cantly increased in hypoxia＋high fat group. Steatosis, inflammatory infiltration and fibrosis was observed in liver tissues of hy-poxia＋ high fat group. Conclusion Intraperitoneal injection of sodium nitrite can establish a hypoxia- induced non- alcoholic steatohepatitis animal model on the basis of high fat diet.