经皮穿刺纤维环建立兔腰椎间盘退变模型

目的探讨使用自制穿刺针经皮穿刺纤维环制备兔腰椎间盘退变模型的可行性。方法将18只新西兰大白兔分为实验组、假手术组及空白对照组。实验组及假手术组使用自制穿刺针穿刺L_(3~4)、L_(4~5)和L_(5~6)椎间盘位置,实验组穿刺椎间盘深度为5 mm,假手术组钝性穿刺但不损伤椎间盘,空白对照组不作处理。术后3、6、9周每组取2只兔麻醉后行腰椎MRI检查,处死行大体观察并取椎间盘行HE染色及髓核蛋白多糖含量测定。结果术后3周开始实验组MRI信号强度、髓核蛋白多糖含量与其他两组相比明显下降(P0.05),其后呈逐渐下降趋势,大体观察及HE染色显示实验组髓核及纤维环呈逐渐退变趋势。结论自制穿刺针经皮穿刺纤维环法能成功建立兔腰椎间盘退变模型,具有操作简单、损伤小、动物存活率高等优点。

Establishment of the intervertebral disc degeneration model of rabbits by using homemade puncture needles percutaneous puncturing the anulus fibrosus at surface allocation

Objective To establish of rabbit intervertebral disc degeneration models by using homemade puncture needles percutaneous annulus fibrosus puncture and assess the feasibility and controllability. Methods 18 New Zea-land white rabbits were divided into the experimental group, the sham group and the control group. L3~4 , L4~5 and L5~6 discs were punctured by the homemade puncture needles in the experimental group as well as in the sham group. The discs were punctured in the experimental group with a depth of 5mm. The sham group was treated by puncture without damage the discs. 2 rabbits were used to observe the anatomical structures. Two rabbits were anesthetized in 3, 6 and 9 weeks respectively after operation for MRI images. Then the animals were killed, the change of lumbar in-tervertebral discs was observed by MRI, histological and nucleus proteoglycan contents were measured. Results All animals were survived, no death, infection, paralysis and other complications. Signal intensity of the intervention group showed a progressive downtrend at 3 weeks after intervention (P<0. 05). General view showed that the nucle-us pulposus and annulus of percutaneous group had a trend of gradual degeneration. Conclusions The experiment may provide a simple lumbar intervertevral disk cataplasia modeling methods that is with small damage, small chances of infection, high survival rate and short operation time.