葛根保肝茶对醋氨酚致小鼠急性肝损伤的保护作用

目的：探讨葛根保肝茶（GGC）对小鼠药物性肝损伤的保护作用，为研究GGC的药理作用提供实验依据。方法：ICR小鼠30只，随机分为空白组、维生素C组和GGC（100 mL?kg^-1）组，每组10只，各组小鼠灌胃给药，每天1次，连续3 d。末次给药后1 h，所有小鼠灌胃给予醋氨酚1 000 mg?kg^-1，醋氨酚致急性中毒24 h后记录各组小鼠死亡情况，计算死亡率。ICR小鼠60只，随机分为空白组、模型组、维生素C组及GGC低、中、高剂量组，空白组和模型组小鼠以蒸馏水灌胃，维生素C组小鼠以1 000 mg?kg-1维生素C灌胃，GGC低、中、高剂量组小鼠以50 、100、200 mL?kg^-1的GGC灌胃，每天1次，连续7 d。末次给药5 h后，除空白组外其他各组小鼠均一次性灌胃醋氨酚500 mg?kg-1，24 h后摘眼球取血，分离血清，测定天门冬氨酸氨基转氨酶（AST）及丙氨酸氨基转氨酶（ALT）水平；称取肝脏，计算肝系数；制备病理切片，HE染色观察病理学改变。制备肝组织匀浆，黄嘌呤氧化法测定超氧化物歧化酶（SOD）活性，改良的硫代巴比妥酸法测定丙二醛（MDA）活性。结果：空白组小鼠死亡率为80%，维生素C组为40%，GGC组为50%，维生素C与GGC对醋氨酚致小鼠死亡的保护率分别为50%和40%。与空白组比较，模型组小鼠肝系数增大（P<0.01）；与模型组比较，GGC中、高剂量组小鼠肝系数降低（P<0.05）。与模型组比较，GGC各剂量组小鼠血清ALT、AST水平和MDA活性均降低（P<0.05），SOD活性升高（P<0.05或P<0.01）。HE染色，模型组小鼠肝细胞水肿性病变，肝脏呈大面积炎症浸润；GGC各剂量组小鼠随GGC剂量的增加，肝脏炎症浸润显著减轻，以200 mg?kg-1剂量组改善效果最明显。结论： GGC对醋氨酚所致的药物性肝损伤具有较强的抑制作用，且随剂量增加保护效果更明显。

Protective effects of Gegencha on acute liver injury induced by acetaminophen in mice

Objective To study the protective effect of gegecha(GGC) on liver injury in mice and to provide experimental basis for study on pharmacological effects of GGC.Methods 30 ICR mice were randomly divided into blank group,vitamin(VC) group and GGC group(100 mL·kg-1),and all of the mice were treated once a day for 3 d.1 h after the last administration,all mice were intragastrically given with acetaminophen 1 000 mg·kg-1.24 h later,the number of dead mice was recorded in each group and the mortality was calculated.Another 60 ICR mice were randomly divided into control group,model group,VC group,and low,middle,and high doses of GGC groups,and there were 10 mice in each group.The mice in control group and model group were intragastrically given with distilled water,the mice in VC group were intragastrically given with VC(1 000 mg·kg-1),the mice in GGC groups were intragastrically given with different doses of GGC(50,100,200 mL·kg-1).All of the mice were treated once a day for 7 d.5 h after the last administration,the mice were intragastrically given with 500 mg·kg-1 acetaminophen,except control group;24 h later,all of the mice were killed and the serum levels of aspartate aminotransferase(AST) and alanine aminotransferase(ALT) were measured according to kit introduction.The liver weights of donor were weighed,and the pathological changes of liver tissues were examined by HE staining method.The activity of superoxide dismutase(SOD) was measured by xanthin oxidase method and the content of malondialdehyde(MDA) was measured by thiobarbituric acid method.Results The mortality in blank group was 80%,40% in VC group,and 50% in GGC(100 mL·kg-1) group.The protective rates of VC and GGC in dead mice induced by acetaminophen were 50% and 40%,respectively.The liver quotient of mice in model group was increased compared with control group(P<0.01),and the liver quotients of mice in middle and high doses of GGC group were lower that in control group(P<0.05).Compared with model group,the serum AST and ALT levels in mice with liver injury were significantly reduced in GGC groups,and showed a significant dose-dependent relationship.Compared with model group,the serum levels of AST,ALT and MDA were decreased(P<0.05),the SOD activities were increased in GGC groups(P<0.05 or P<0.01).The HE staining results showed that liver cells were swelling and there was a large area of inflammatory infiltration in model group,while in GGC groups,the liver inflammatory infiltration was reduced with the increasing of GGC dose,and the effect of 200 mg·kg-1 GGC was most obvious.Conclusion GGC has significantly protective effect on liver tissues in mouse liver injury models induced by acetaminophen,and the protective effect of GGC on liver tissues is more obvious with the increasing of GGC dose.