| 细粒棘球蚴生发细胞特异性抗原的ELIB分析 |
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| 引用本文： | 冯建军,汪俊云,等.细粒棘球蚴生发细胞特异性抗原的ELIB分析[J].中国寄生虫学与寄生虫病杂志,1993,11(1):63-65. |
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| 作者姓名： | 冯建军 汪俊云 |
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| 作者单位： | [1]中国预防医学科学院寄生虫病研究所世界卫生组织疟疾血 [2]中国预防医学科学院寄生虫病研 |
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| 摘 要： | 应用SDS－PAGE和免疫印渍试验（EITB），证明体外培养的细粒棘球蚴生发细胞和囊液含有特异性抗原，分子量为52kDa和38kDa，而囊壁则含有此2种抗原。生发细胞的52kDa抗原可识别感染小鼠和包虫病患者血清的特异性抗体，对囊虫病人和正常人血清则无反应条带。
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| 关 键 词： | 棘球属 体外培养 抗原 抗体 ELIB |
ELIB OF SPECIFIC ANTIGENS IN IN VITRO CULTIVATED GERMINAL CELLS OF ECHINOCOCCUS GRANULOSUS |
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| Feng Jian-Jun,Wang Jun-Yun,Qu Jing-Qi,Xiao Shu-HuaInstitute of Parasitic Diseases,Chinese Academy of Preventive Medicine, WHO Collaborating Centre forMalaria,Schistosomiasis and Filariasis,Shanghai.ELIB OF SPECIFIC ANTIGENS IN IN VITRO CULTIVATED GERMINAL CELLS OF ECHINOCOCCUS GRANULOSUS[J].Chinese Journal of Parasitology and Parasitic Diseases,1993,11(1):63-65. |
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| Authors: | Feng Jian-Jun Wang Jun-Yun Qu Jing-Qi Xiao Shu-HuaInstitute of Parasitic Diseases Chinese Academy of Preventive Medicine WHO Collaborating Centre forMalaria Schistosomiasis and Filariasis Shanghai |
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| Affiliation: | Feng Jian-Jun,Wang Jun-Yun,Qu Jing-Qi,Xiao Shu-HuaInstitute of Parasitic Diseases,Chinese Academy of Preventive Medicine, WHO Collaborating Centre forMalaria,Schistosomiasis and Filariasis,Shanghai 200025 |
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| Abstract: | Specific antigens are detected in cyst fluid and cyst wall of Echinococcus granulosus, as well as germinal cells cultivated in vitro by sodium dodecyl sulphate polyacrylamide gel elec-trophoresis (SDS-PAGE) and enzyme linked immunotransfer blot technique (ELIB), using the sera from mice infected with protoscoleces of E. granulosus for at least 10 months. A specific reaction band of 52 kDa or 38 kDa was detected in soluble protein of germinal cells and cyst fluid, respectively, but these two reaction bands were present concurrently in the cyst wall. The sera from 7 normal controls, 7 hydatidosis patients and 3 cysticercosis patients were used to study the specific antigen from the germinal cells. The results noted that the reaction band of 52 kDa was seen in all the sera from hydatidosis patients, while the sera from normal human controls and cysticercosis patients showed no reaction band. |
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| Keywords: | Echinococcus germinal cell in vitro culture enzyme linked immunotransfer blot technique antigen antibody |
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