| 益气通络丹抗缺氧再给氧心肌细胞凋亡的实验研究 |
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| 引用本文： | 沈剑刚,佟丽,邱幸生,曲宏达,陈育尧.益气通络丹抗缺氧再给氧心肌细胞凋亡的实验研究[J].南方医科大学学报,2001,21(10):743-745. |
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| 作者姓名： | 沈剑刚 佟丽 邱幸生 曲宏达 陈育尧 |
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| 作者单位： | 第一军医大学中医系 |
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| 基金项目： | 国家自然科学基金(39300178)，广东省自然科学基金(95056719) |
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| 摘 要： | 目的观察含益气通络丹的大鼠血清对缺氧24h再给氧4h后新生乳鼠心肌细胞凋亡的抑制作用及其对心肌细胞乳酸脱氢酶(LDH)释放、一氧化氮(NO)生成和硫代巴比妥酸反应物质(TBARS)的影响。方法采用AnnexinV-PI双标记方法以流式细胞仪和ELISA法检测细胞凋亡,以紫外分光光度法测定LDH释放水平,采用改良Yu方法测定NO浓度,用Ohkawa法测定TBARS含量。结果心肌细胞缺氧再给氧后NO、TBARS水平升高,LDH释放增加。含益气通络丹的血清能显著抑制心肌细胞凋亡,抑制LDH释放,降低培养细胞上清液中NO和TBARS水平,其效应呈剂量依赖关系。结论益气通络丹具有抗缺氧再给氧心肌细胞凋亡作用,其机制与清除氧自由基和NO特性有关。
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| 关 键 词： | 补气剂 供氧 通络 脱噬作用 心肌 心肌再灌注 |
| 文章编号： | 1000-2588(2001)10-0743-03 |
| 修稿时间： | 2001年5月15日 |
Yiqitongluo pellet inhibits cardiomyocyte apoptosis induced by reoxygenation following hypoxia: an experimental observation |
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| SHENJian-gang,TongLi,QIUXing-sheng,QUHong-da,CHENYu-yao.Yiqitongluo pellet inhibits cardiomyocyte apoptosis induced by reoxygenation following hypoxia: an experimental observation[J].Journal of Southern Medical University,2001,21(10):743-745. |
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| Authors: | SHENJian-gang TongLi QIUXing-sheng QUHong-da CHENYu-yao |
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| Abstract: | Objective To investigatethe inhibitoryeffectof Yiqitongluopelleton culturedneonatalrat cardiomyocyte apoptosisinducedby reoxygenationwithprecedinghypoxiaandobservethechangesin thelevelsof lactabledehydrogenase,nitricoxide(NO)andthiobarbituricacid-reactivesubstances(TBARS)in theinoculum.Methods The neonatalrat cardiomyocyteswereobtainedto establishmodelsof reoxygenationfollowinghypoxia,andsubsequentlytreatedwiththe serumisolatedfrom Yiqitongluopellet-fedWistarratsadministeredbeforeannexinV-PIlabeling.Flowcytometryand enzyme-linkedimmunosorbentassaywereperformedfor cellularapoptosisassessment.The concentrationof LDHin the inoculumwasmeasuredwithspectrophotometer,andimprovedYu methodandOhkawamethodwereemployedto determine NO and TBARSlevels,respectively.Results Yiqishuluopelletratserumsignificantlyinhibitedthecardiomyoctesfrom apoptosisinducedby reoxygenationwithprecedinghypoxia,and the elevatedNO,LDH and TBARSlevelsdue to reoxygenationintheinoculumwerebroughtdownby themedication-containingserum.Conclusion Yiqitongluocaninhibite cardiomyocyteapoptosisinducedby reoxygenationfollowinghypoxiaby themechanisminvolvingtheeliminationof oxygen freeradicalsandNOproductionregulation. |
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| Keywords: | reinforcingagent anoxia dredgingcollaterals apoptosis myocardium myocardialreperfusioninjury |
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