膀胱移行细胞癌MMP-9表达与间质微血管密度、肿瘤转移的关系

目的:研究基质金属蛋白酶-9(MMP-9)在膀胱移行细胞癌组织中的表达特点及其与微血管密度(microvessel density,MVD)、肿瘤转移的关系。方法:应用S-P免疫组化法检测54例膀胱移行细胞癌、10例癌旁正常组织MMP-9的表达,并在CD34染色切片上检测问质MVD。结果:膀胱移行细胞癌组织MMP-9的表达(62.96％)显著高于癌旁正常组织(20％),两者之问差异有显著性(P<0.05);MMP-9阳性组MVD均值(38.51±14.36)高于MMP-9阴性组(28.12±11.37),MMP-9的表达与MVD成正相关(P<0.01)。此外,膀胱移行细胞癌MMP-9的表达与组织学分级、大小、血管淋巴管的转移密切相关(P<0.05),而与患者年龄、临床分期无关。结论:MMP-9促进膀胱移行细胞癌间质血管生成,促进肿瘤的侵袭和转移,有可能成为判定膀胱移行细胞癌生物学行为和预后重要指标。

Expression of matrix metalloproteinase-9 and its correlation with tumor interstitial microvascular density and tumor metastasis in transitional cell carcinoma of the bladder

Purpose: To study the expression of matrix metalloproteinase-9 ( MMP-9) and its correlation with interstitial microvascular density(MVD) and tumor metastasis in transitional cell carcinoma of the bladder ( TCCB). Methods: The expression of MMP-9 and MVD were detected in 54 cases of TCCB and 10 cases of normal tissue near the tumor by immunohistochemical technique. Results: In comparison with the above non-tumor normal tissue (20%), the expression of MMP-9 in TCCB (62. 96%) was significantly higher (P < 0. 05). MVD was much higher in the group of MMP-9 positive expression than that in the group of MMP-9 negative expression, there was a positive correlation between MMP-9 and MVD(P <0. 05). In addition, the expression of MMP-9 was correlated with histological grade, tumor size and lymph node metastasis (P<0. 05), but there was no relationship with age and clinical stage. Conclusions: MMP-9 may promote angiogenesis and play an important role in tumor invasion and metastasis. MMP-9 may be an important indicator to determine biological behavior and prognosis of TCCB.