Characterization of phage-specific transfer RNA molecules coded by Vibrio eltor phage e4 |
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Authors: | S Chattopadhyay R K Ghosh |
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Affiliation: | Indian Institute of Chemical Biology, Calcutta. |
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Abstract: | Transfer RNAs were isolated from phage e4-infected Vibrio eltor Mak 757 cells. These were aminoacylated with 14 individual 3H-labeled L-amino acids. Hybridization of these 3H]aminoacyl-tRNAs with phage e4 DNA revealed that the phage e4 encodes tRNAs for arginine, tryptophan, tyrosine, leucine, and isoleucine. Direct aminoacylation of phage-coded tRNA molecules isolated from phage DNA-RNA hybrids also confirmed this observation. |
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