| 高分辨分型方法分析福建地区HLA-B15组的基因多态性 |
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| 引用本文： | 杨凤娥,金静君,陈志哲,付丹晖,赖力.高分辨分型方法分析福建地区HLA-B15组的基因多态性[J].福建医药杂志,2010,32(5):75-76. |
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| 作者姓名： | 杨凤娥 金静君 陈志哲 付丹晖 赖力 |
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| 作者单位： | 1. 福建医科大学附属协和医院,福州,350003
2. 福建省医学科学研究院 |
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| 基金项目： | 福建省教育厅科研项目 |
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| 摘 要： | 目的研究福建地区HLA-B15组等位基因的分布特点,探讨其可能对临床供者选择的影响。方法用聚合酶链反应序列特异引物（PCR-SSP）方法,从已分型的300例供者中选出低分辨率结果为B＊15的样本进行高分辨率分型。结果共检出6种HLA-B15的等位基因,分属于4种血清学特异性。结论福建人群B＊15基因组中B＊1501占优势,其次为B＊1502。为选择最适供者,建议临床配型中采用高分辨率基因分型。
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| 关 键 词： | HLA-B基因 高分辨率分型 基因多态性 PCR-SSP |
Polymorphism analysis of HLA-B15 group in Fujian with high resolution method |
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| YANG Feng-e,JIN Jing-jun,CHEN Zhi-zhe,FU Dan-hui,LAI Li.Polymorphism analysis of HLA-B15 group in Fujian with high resolution method[J].Fujian Medical Journal,2010,32(5):75-76. |
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| Authors: | YANG Feng-e JIN Jing-jun CHEN Zhi-zhe FU Dan-hui LAI Li |
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| Affiliation: | .The Affiliated Union Hospital,Fujian Medical University,Fuzhou 350001,China |
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| Abstract: | Objective To study the Polymorphism of HLA-B15 group and to explore its effect on transplantation donor selection.Methods The HLA-B genetypes of 300 individuals selected from Fujian marrow donors were identified by PCR-SSP,and then the HLA-B15 positive samples from above population were analyzed by PCR-SSP at high resolution.Results There were six alleles of HLA-B15 group were detected,and they covered four kinds of serological specificity.Conclusion The predominant allele in HLA-B15 gene family is B1501.The high-resolution typing is recommended for the selection of clinical transplantation donor. |
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| Keywords: | PCR-SSP |
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