益生菌对炎症性肠病小鼠肠道菌群紊乱及细菌移位的影响

目的探讨植物乳杆菌（LP）对炎症性肠病（IBD）小鼠肠道菌群及细菌移位的影响。方法采用白介素10基因敲除（IL-10^-/-）小鼠作为IBD动物模型，将8周龄雌性小鼠随机分成空白对照组、IL-10^-/-组和IL—10^-/- ＋LP组三组。IL-10^-/- ＋LP组每日灌胃0．5mLLP菌液（1．0×10^9 CFU／mL），其余两组灌胃Ringer缓冲液0．5mL，持续4周。以小鼠粪便中的双歧杆菌、乳酸杆菌、肠杆菌和产气荚膜梭菌数量及肠系膜淋巴结、脾脏细菌移位为检测指标。结果IL-10^-/-小鼠肠内双歧杆菌、乳酸杆菌含量明显下降，肠球菌、产气荚膜梭菌含量升高，且肠道细菌移位明显增加；而连续灌胃LP菌液4周后，益生菌发挥了对肠道的调节作用，纠正了肠道菌群失衡，并降低了肠道细菌移位。结论LP能纠正炎症性肠病小鼠肠道菌群紊乱，减少细菌移位，从而增强了肠道屏障功能。

Effect of probiotics on intestinal flora disturbance and bacterial translocation in mice with spontaneous colitis

Objective To investigate the effect of Lactobacillus plantarum(LP)on intestinal flora and bacterial translocation in mice with spontaneous inflammatory bowel disease(IBD). Methods Interleukin 10 knockout mice(IL-10~(-/-))were used as models of IBD.Eight-week old female mice were randomized to control group, IL-10~(-/-)group and IL-10~(-/-)+LP group.IL-10~(-/-)+LP group received 0.5 mL LP(1.0×10~9CFU/mL)per day for 4 weeks,and the other groups received 0.5 mL Ringer buffer.Intestinal flora including Bifidobacteria,Lactobacilli,Enterobacteriaceae and Clostridium perfringens in the feces and bacterial translocation in mesenteric lymph nodes and spleens were detected. Results The contents of Bifidobacteria and Lactobacilli significantly decreased in the intestine of IL-10~(-/-)mice,while those of Enterobacteriaceae and Clostridium perfringens significantly increased,and the bacterial translocation significantly increased.Four weeks after LP treatment, the disturbed intestinal flora was restored, and the bacterial translocation decreased. Conclusion LP administration can modulate the imbalance of intestinal flora and decrease the bacterial translocation,thus enhance intestinal barrier function in mice with IBD.