高效液相色谱法测定浙江平阳栀子熊果酸含量

目的:为控制浙江平阳栀子的质量,建立高效液相色谱法测定浙江平阳栀子中熊果酸含量的方法。方法:采用高效液相色谱法,以熊果酸为化学对照品,固定相:UltimateTMC18键合硅胶柱(4.6mm×250mm,5μm),流动相:甲醇:水:冰醋酸:三乙胺(90∶10∶0.03∶0.06),流速:0.6mL.min-1,温度:25℃,波长:210nm测定浙江平阳栀子熊果酸含量。结果:熊果酸在0.1~3.0μg范围内峰面积A与进样量M呈现良好的线性关系,其回归方程为:A=-12954.8829+452850.9603M,γ=0.9996,n=7;三批浙江平阳栀子中熊果酸的含量分别为(0.586±0.010)%,RSD%=1.72,n=3;(0.537±0.020)%,RSD%=3.689,n=4;(0.497±0.012)%,RSD%=2.405,n=4;精密度试验RSD%=1.754,仪器精密度良好;稳定性试验RSD%=3.493,在3.5h内测定熊果酸的含量是稳定的;重复性试验RSD%=2.849,平均回收率为101.9%。结论:高效液相色谱法适合浙江平阳栀子中熊果酸的含量测定,该方法操作简便,结果稳定,精密度、重现性、回收率较好,值得推广。

Determination of the Content of Ursolic Acid in Fructus Gardeniae by HPLC: Natural drug from Pingyang county in Zhejiang province

Objective:To control the quality of Fructus Gardeniae from Pingyang county in Zhejiang province,we established the method of determining the cotent of Ursolic acid in Fructus Gardeniae by HPLC.Methods:Ursolic acid as the standard,Solid phase:UltimateTM C18(4.6mm×250mm,5μm),Liquid phase:Methyiaicohol:Water:Acetic acid:Triethylaminum(90∶10∶0.03∶0.06),Flow rate:0.6mL·min-1 temperature:25℃,wavelength:210nm,we used HPLC to determine the content of Ursolic acid in Fructus Gardenice.Results:The calibration curve is very good linear in the range of 0.1~3.0μg,and the return formula is A=-12954.8829+452850.9603M,r=0.9996,n=7,the contents of Ursolic acid in Frctus Gardeni are(0.586±0.010)%,RSD%=1.72,n=3;(0.537±0.020)%,RSD%=3.689,n=4;(0.497±0.012)%,RSD%=2.405,n=4;respectively.precision is RSD%=1.754,stablity RSD%=3.493,repeatability RSD%=2.849,recovery 101.9%.Conclusion:HPLC is suitable for the determination of Ursolic acid in Fructus Gardeniae from Pingyang county in Zhejiang province.This method operated convenientely,the result is stable and has good repeatability,recovery and precision.It can be widely used for the quality control of Fructus Gardeniae.