| PADAM动物模型进行同种异体睾丸间质细胞移植的研究 |
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| 引用本文： | 孙祥宙,邓春华,郭海彬,刘建中.PADAM动物模型进行同种异体睾丸间质细胞移植的研究[J].中国男科学杂志,2005,19(3):9-11. |
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| 作者姓名： | 孙祥宙 邓春华 郭海彬 刘建中 |
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| 作者单位： | 中山大学附属第一医院泌尿外科,广州,510080 |
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| 基金项目： | 广东省科技计划项目基金(2003C34008)，广州市市科委基金(2000J-002.02)，广东省医学科研基金(B2004034)资助 |
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| 摘 要： | 目的老年SD大鼠进行同种异体睾丸间质细胞移植探讨中老年男性雄激素部分缺乏症(PADAM)动物模型的可行性和疗效。方法选10只符合PADAM模型标准的老年SD大鼠作为受体，用成年SD大鼠的睾丸进行体外分离和培养，将获得的高纯度和高活力的睾丸间质细胞移植到老年SD大鼠的大腿内侧肌群内，定期检查其移植前后血清睾酮和游离睾酮的水平变化，并观察移植部位的情况。结果未应用免疫抑制剂，移植后的睾丸间质细胞保持良好的分泌功能，老年SD大鼠的血清睾酮和游离睾酮水平均显著升高，并大约于移植后的7～12d开始稳定在一定的水平，可持续27d以上。移植部位未见异常。结论睾丸问质细胞同种异体移植安全、有效、无明显排斥反应。
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| 关 键 词： | 中老年男性雄激素部分缺乏症 睾丸间质细胞 移植 睾酮 游离睾酮 |
| 修稿时间： | 2004年7月13日 |
Experimental study of allograft Leydig cells transplantation of animal model of PADAM |
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| Sun Xiangzhou,Deng Chunhua,Guo Haibin,Liu Jianzhong.Experimental study of allograft Leydig cells transplantation of animal model of PADAM[J].Chinese Journal of Andrology,2005,19(3):9-11. |
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| Authors: | Sun Xiangzhou Deng Chunhua Guo Haibin Liu Jianzhong |
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| Affiliation: | Sun Xiangzhou,Deng Chunhua,Guo Haibin,Liu Jianzhong Department of Urology,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China |
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| Abstract: | Objective To investigate the feasibility of Leydig cells transplantation in aged SD rat (the animal model of PADAM). Methods Ten aged SD rats, whose serum testosterone and free testosterone were significantly decreased, were selected as the model of PADAM. Leydig cells of adult rat testes were cultured in vitro and transplanted into the vastus medialis of the aged SD rats. The serum testosterone and free testosterone of the aged SD rats were monitored after transplantation. Results After transplantation, the Leydig cells worked well in the receptor, the serum testosterone and free testosterone were increased significantly, and it maintained in a high level after 7 to 12 days of transplantation. Conclusion Allograft Leydig cells transplantation is an ideal treatment for testosterone replacement. |
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| Keywords: | partial androgen deficiency in aging male Leydig cells transplantation testosterone free testosterone |
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