| 硒对环氧化酶2 mRNA表达的影响 |
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| 引用本文： | 王舒然,杨宝峰,孙长颢,暴永平.硒对环氧化酶2 mRNA表达的影响[J].中国公共卫生,2004,20(5):570-571. |
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| 作者姓名： | 王舒然 杨宝峰 孙长颢 暴永平 |
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| 作者单位： | 1. 哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,哈尔滨,150001
2. 英国食品研究所 |
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| 摘 要： | 目的 观察硒对肿瘤发生密切相关的环氧化酶2(COX-2)表达的影响。方法 选用人结肠癌细胞系HT-29，采用定量PCR方法测定不同剂量(0.25，0.5，1.0μmol/L)在4，8和24h对COX-2mRNA表达的影响。结果 硒在0.5和1.0μmol/L剂量作用24h条件下，对HT-29的COX-2mRNA表达有下调作用，其中0.50μmol/ L是最佳作用剂量。结论 硒通过下调COX-2mRNA表达，发挥癌症预防作用。为科学饮食预防癌症和将硒作为抗癌药物进行开发提供理论依据。
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| 关 键 词： | 硒 环氧化酶-2（COX-2） 定量PCR |
| 文章编号： | 1001-0580(2004)05-0570-02 |
| 修稿时间： | 2004年2月12日 |
Effects of Se on COX- 2 mRNA expression |
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| WANG Shu ran,YANG Bao feng,SUN Chang hao,et al..Effects of Se on COX- 2 mRNA expression[J].Chinese Journal of Public Health,2004,20(5):570-571. |
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| Authors: | WANG Shu ran YANG Bao feng SUN Chang hao |
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| Affiliation: | WANG Shu ran,YANG Bao feng,SUN Chang hao,et al.Department of Nutrition and Food Hygiene,Harbin Medical University |
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| Abstract: | Objective To investigate effects of Se,in the form of sodium selenite,on COX-2 mRNA expression,a cancer close related gene.Methods The amount of COX-2 mRNA of HT 29 cells,which were cultured in DMEM containing the concentration of 250,500 and 1?000?nmol/L Se for 4,8 and 24?h,was detected by real time PCR.Results The expression of COX-2 mRNA were down regulated significantly by 500 and 1?000?nmol/L Se for 24?hours.Conclusion Se had chemoprotective effect on human colon cancer through down regulating COX-2 gene expression and did good to man's suitable diet and offer a background for develoing Se anticancer drug. |
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| Keywords: | Se COX-2 real time PCR |
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