阔叶山麦冬总皂苷对D-半乳糖衰老小鼠学习记忆及代谢产物的影响

目的:探讨阔叶山麦冬总皂苷(Liriope platyphylla total saponin,LPTS)对D-半乳糖所致衰老小鼠学习记忆和神经介质相关酶及代谢产物的影响。方法:90只昆明种小鼠随机分为9组:生理盐水(normal saline,NS)组,模型组,LPTS高(100 mg/kg)、中(50 mg/kg)、低(10mg/kg)剂量组,舒血宁组,绞股蓝总苷组,西比灵组,维生素E组,除NS组外,其余各组每天腹腔注射80 mg/kg D-半乳糖生理盐水溶液,然后小鼠再给予相应的药物干预,连续给药造模42d后,分别检测相关衰老指标。结果:LPTS对D-半乳糖衰老小鼠学习记忆障碍有一定的改善作用,并且可以显著促进衰老小鼠体质量增长,提高其胸腺系数和脾系数,降低血清丙二醛的含量,降低脑组织单胺氧化酶活性和脂褐质水平,提高血清超氧化物歧化酶活性,升高肝谷胱甘肽含量。结论:LPTS可改善学习记忆障碍,并且具有抗衰老的作用。

Effect of Liriope platyphylla total saponin on learning,memory and metabolites in aging mice induced by D-galactose

Objective:To investigate the effects of Liriope platyphylla total saponin(LPTS)on learning,memory. neuromediators and metabolites in aging mice induced by D-galactose. Methods:Ninety Kunming mice were randomly divided into nine groups:normal saline(NS)-treated group, untreated group,high-(100 mg/kg),medium-(50 mg/kg)and low-dose(10 mg/kg)LPTS-treated groups. Shuxuening-treated group,jiaogulanosidi-treated group,flunarizine-treated group and vitamin E-treated group.The Kunming mice in the NS-treated group were administered with NS by intraperitoneal injection. while the aging mice in the other eight groups were administered with D-galactose by intraperitoneal injection. At the same time.the aging mice in different groups were fed with corresponding drugs for 42 days.and the aging items of the mice in different groups were measured,respectively. Results:LPTS could improve the memory of aging mice induced by D-galactose,promote its body weight. and increase the thymus and spleen indexes of the aging mice.LPTS could decrease the levels of MDA and lipofuscin,inhibit MAO activity and increase SOD activity and GSH-Px level. Conclusion;LPTS may improve the ability of learning and memory and delay aging.