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| 刺五加抗疲劳活性部位中刺五加苷B的含量测定 | |
| 引用本文： | 李辰,王晓燕,胡绪玮,范海涛,乔善义.刺五加抗疲劳活性部位中刺五加苷B的含量测定[J].中国中药杂志,2008,33(23):2800-691. |
| 作者姓名： | 李辰 王晓燕 胡绪玮 范海涛 乔善义 |
| 作者单位： | 1. 沈阳药科大学,辽宁,沈阳,110016 2. 军事医学科学院,毒物药物研究所,北京,100850 |
| 基金项目： | 全军医药卫生科研基金B类 |
| 摘 要： | 目的：建立刺五加抗疲劳活性部位中刺五加苷B的含量测定方法。方法：用小鼠负重游泳实验，确定刺五加粗提物的抗疲劳活性部位；硅胶柱色谱，Sephadex LH-20柱色谱，制备液相色谱对活性部位分离纯化，并对分离所得化合物进行理化性质研究和结构鉴定；含量测定中高效液相色谱条件为Diamonsil C18柱(4.6 mm×250 mm,5 μm)，流动相乙腈-水-醋酸(10∶90∶0.01)，流速1.0 mL·min-1，紫外检测波长344 nm。结果：从刺五加抗疲劳活性部位As1中分离得到一化合物，鉴定为刺五加苷B；刺五加苷B的线性范围为 0.104～20.8 μg，r=0.999 9；平均回收率为97.68%，RSD 1.4%(n=6)。结论：从刺五加抗疲劳活性部位As1中分离得到含量较高的刺五加苷B，作为质量控制的指标成分，并建立了刺五加抗疲劳活性部位中刺五加苷B含量测定方法。 |
| 关 键 词： | 刺五加 抗疲劳 刺五加苷B 高效液相色谱 |
| 收稿时间： | 1/1/1900 12:00:00 AM |
| 修稿时间： | 1/1/1900 12:00:00 AM |
Determination of eleutheroside B in antifatigue fraction of Acanthopanax senticosus by HPLC |
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| LI Chen; WANG Xiao-yan; HU Xu-wei; FANG Hai-tao; QIAO Shan-yi.Determination of eleutheroside B in antifatigue fraction of Acanthopanax senticosus by HPLC[J].China Journal of Chinese Materia Medica,2008,33(23):2800-691. |
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| Authors: | LI Chen; WANG Xiao-yan; HU Xu-wei; FANG Hai-tao; QIAO Shan-yi |
| Affiliation: | 1. Shenyang Pharmaceutical University, shenyang 110016, China; 2. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China |
| Abstract: | Objective: To develop an HPLC method for the determination of eleutheroside B in As1(the extract fraction from Acanthopanax senticosus,which has a good effect of antifatigue).Method: The antifatigue effect of As1 was evaluated by mice burden swimming testing.One compound was isolated by silica gel,Sephadex LH-20 column chromatography and preparative high performance liquid chromatography.The structure was identified by physicochemical properties and spectral evidences.Eleutheroside B in As1 was determined b... |
| Keywords: | Acanthopanax senticosus antifatigue eleutheroside B HPLC |
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