不同来源莪术中姜黄素类成分的含量差异研究

目的：用高效液相色谱法测定不同来源的莪术中3种姜黄素成分，比较姜黄素（cur_cumin，Ⅰ）、去甲氧基姜黄素（demethoxycurcumin，Ⅱ）和双去甲氧基姜黄素（bisdemethoxycurcum_in，Ⅲ）的含量差异。方法：采用Kromasil色谱柱,流动相为乙腈-水（0.5%冰乙酸）,流速为1 mL· min-1，检测波长为425 nm。结果：姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素的线性范围分别为0.04~0.16μg （r=0.9991）、0.037~0.150μg （r=0.9990）、0.00015~0.00062μg （r=0.9996）；平均回收率（n=9）分别为103.32%、100.32%、100.06%。结论：不同来源的莪术中姜黄素类成分的含量存在着明显的差异。

Research on Contents of Curcuminoids in Different Curcuma Samples

Objective： To research on the content differences of curcuminoids including curcumin, demethoxycurcumin and bisdemethoxycurcumin in Rhizoma Curcumae from different sources. Methods： A reversed-phase HPLC system consisting of an ODS column and a mixture of acetonitrle-water （with 0.5%acetic acid） as the mobile phase was used. The flow rate was 1.0 mL·min-1 and the detection was effected at 425 nm. Results： The calibration curves of curcumin, demethoxycurcumin and bisdemethoxycurcumin were linear in the range of 0.04~0.16μg （r=0.9991）, 0.037~0.150μg （r=0.9990）, 0.00015~0.00062μg （r=0.9996）, respectively; The average recoveries （n=9） were 103.32%, 100.32% and 100.06%, respectively. Conclusion： The results showed that the contents of curcuminoids vary with sample source.