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Studies on the production of anthraquinone derivatives by tissue culture ofRubia species
Authors:Soon-Hee Shin
Affiliation:1. College of Pharmacy, Duksung Women’s University, 132-714, Seoul, Korea
Abstract:The tissue culture ofRubia cordifolia var.pratensis andR. akane were performed to enhance the biosynthesis of anthraquinone pigments under various conditions. The production of alizarin and purpurin in the callus was separately analysed and was quantitatively compared. The pigment biosynthesis was more active in the callus fromR. cordifolia var.pratensis than fromR. akane. The addition of α-ketoglutaric acid, a biosynthetic precursor of anthraquinones, enhanced the production of alizarin and purpurin remarkably.
Keywords:Rubia cordifolia var  pratensis                      Rubia akane            tissue culture  alizarin  purpurin  natural dyestuffs  phytohormone
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