miR - 187 影响结肠癌 LOVO 细胞恶性生物学行为的研究简

目的：探讨miR-187对结肠癌LOVO细胞增殖、凋亡、侵袭转移等恶性生物学行为的影响。方法：应用miR-187mimics转染结肠癌LOVO细胞，利用MTT实验、流式细胞术和Transwell实验观察miR-187对结肠癌LOVO细胞增殖、凋亡、侵袭转移的影响。结果：与对照细胞相比，MTT显示转染miR-187的LOVO细胞增殖速度变快，流式细胞术表明miR-187mimics转染可以减少LOVO细胞的凋亡，Transwell实验证实转染miR-187mimics可以促进LOVO细胞迁移，增强LOVO细胞的侵袭转移能力。结论：miR-187促进结肠癌LOVO细胞的恶性增殖、抑制凋亡、促进侵袭和转移，提示miR-187在结肠癌的恶性生物学进程中可能发挥重要作用。

The effect of miR -187 on the malignant behavior of colon cancer LOVO cell

Objective：To investigate the effect of miR -187 on the cell proliferation,cell apoptosis and metastasis of colon cancer LOVO cell. Methods：miR - 187 mimics was transfeeted into LOVO cell. MTT assays, flow cytometry assay,cell invasion and migration assays were performed to evaluate the effect of miR- 187 on the cell proliferation, cell apoptosis and metastasis of colon cancer LOVO cell. Results ： As indicated by MTT assay, compared with control cells, miR - 187 mimics transfected LOVO cells showed a significantly increased rate of cell proliferation. Flow cytometry assay showed a decreased apoptotie index of miR - 187 mimics transfeeted LOVO cells. Cell invasion and migration assays suggested miR- 187 could promote the migration and invasion of LOVO cell in vitro. Conclusion：miR- 187 promotes the proliferation and metastasis of LOVO cells. These results suggest miR - 187 play an important role in the malignant phenotypes of colon cancer.