D101型大孔树脂纯化附子生物碱

目的:研究附子生物碱经D101型大孔树脂富集后化学成分的变化.方法:以附子中总生物碱、酯型生物碱、乌头碱、次乌头碱及新乌头碱的转移率为指标,采用UV,HPLC,TLC分别对富集前后附子提取物中的生物碱进行含量测定及其成分鉴别.结果:采用D101型大孔树脂富集附子生物碱,总生物碱转移率83.70％,纯度67.34％；乌头碱转移率77.78％,次乌头碱转移率94.12％,新乌头碱转移率52.63％；TLC比较发现显示6个相似的生物斑点,说明富集前后生物碱化学成分无明显差异.结论:D101型大孔树脂能有效提高附子中总生物碱的纯度,且各生物碱转移率较高,可用于大生产推广.

Enrichment and Purification of Alkaloids from Aconitum carmichaelii with D101 Macroporous Resin

Objective: To resarch chemical composition change after enrichment and purification of alkaloids from Aconitum carmichaelii with D101 macroporous resin. Method: Taking transfer rate of total alkaloids, diester diterpenoid alkaloids, aconitine,hypaconitine and mesaconitine as indexes, before and after purification,the content of alkaloids from extracts of A. carmichaelii was determined by UV and HPLC,ingredients of alkaloids differentiated by TLC. Result: Alkaloids from A. carmichaelii was enriched by D101 macroporous resin,transfer rate and purity of total alkaloids were 83.70%,67.34%,respectively;Transfer rate of aconitine,hypaconitine and mesaconitine were 77.78%,94.12%,52.63%;It showed 6 similar biological spots by TLC comparison, this indicated that there was no significant difference before and after enrichment of alkaloids composition. Conclusion: D101 type macroporous resin could effectively enhance purity of total alkaloids from A. carmichaelii with high transfer rate of alkaloids,it could be used for production promotion.