| 茜草提取物中大叶茜草素测定的方法研究 |
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| 引用本文： | 浦益琼,王冰,张彤,陶建生,丁越.茜草提取物中大叶茜草素测定的方法研究[J].中国实验方剂学杂志,2012,18(6):94-96. |
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| 作者姓名： | 浦益琼 王冰 张彤 陶建生 丁越 |
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| 作者单位： | 上海中医药大学,上海,201203 |
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| 基金项目： | 教育部新世纪人才计划(NCET-10-0944);上海市曙光计划(10SG40);上海市创新团队及重点学科(J50302) |
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| 摘 要： | 目的:建立茜草提取物中大叶茜草素的HPLC含量测定方法.方法:采用Ultimate XB-C18(4.6 mm×250 mm,5μm),流动相为甲醇-乙腈-0.2％磷酸(25∶50∶25),流速为1 mL·min-1,柱温25℃,检测波长250 nm.结果:在该条件下,大叶茜草素在3.566～ 456.4 mg·L-1线性关系良好(r=0.9999);平均回收率为98.97％( RSD 2.29％).结论:该方法准确、可靠,操作简便,可用于茜草提取物的质量控制方法.
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| 关 键 词： | 茜草 大叶茜草素 高效液相色谱法 |
| 收稿时间： | 2011/10/14 0:00:00 |
The Methodology Study on Mollugin Content Determination in Radix Rubiae Extracts |
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| PU Yi-qiong,WANG Bing,ZHANG Tong,TAO Jian-sheng and DING Yue.The Methodology Study on Mollugin Content Determination in Radix Rubiae Extracts[J].China Journal of Experimental Traditional Medical Formulae,2012,18(6):94-96. |
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| Authors: | PU Yi-qiong WANG Bing ZHANG Tong TAO Jian-sheng and DING Yue |
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| Affiliation: | Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| Abstract: | Objective:To establish a quantitative HPLC method for the determination of mollugin in extract from Rubia cordifolia L. Method:The determination was carried out on a Ultimate XB-C18 column at 25 ℃ with methanol-acetonitrile-0. 2% phosphoric acid ( 25∶50∶ 25) as mobile phase and detection wavelength at 250 nm. The flow rate was 1. 0 mL·min -1. Result:The good linearity was obtained with the correlation coefficients ranged of 3. 566-456. 4 mg · L-1 ( r = 0. 999 9 ) . The average recovery rate was 98. 97% ( RSD 2. 29% ) . Conclusion:This method is accurate and reliable with easy operation,which can be used in the quality control of extracts from R. cordifolia. |
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| Keywords: | Rubia cordifolia L mollugin HPLC |
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