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竹叶中茶多酚的提取及其抗氧化性研究
引用本文:王文渊,黄光文,龙红萍,张芸兰,周金彩,周振华.竹叶中茶多酚的提取及其抗氧化性研究[J].香料香精化妆品,2011(2):24-28.
作者姓名:王文渊  黄光文  龙红萍  张芸兰  周金彩  周振华
作者单位:1. 湖南永州职业技术学院,湖南,永州,425000
2. 湖南科技学院可再生资源研究所再生资源重点实验室,湖南,永州,425007
3. 中南大学药学院,湖南,长沙,410013
摘    要:探讨用超声技术从竹叶中提取茶多酚的最优工艺条件,并对茶多酚的抗氧化性进行研究。以茶多酚的产率为考察指标,通过单因素试验和正交试验确定从竹叶中超声提取茶多酚的适宜工艺条件:超声功率为70W,浸提温度75℃,超声时间40mn,料液比1:20,乙醇体积分数75%,此条件下茶多酚提取产率为0.624300。以Fenton反应、DPPH法检测其清除自由基活性,结果表明,竹叶中茶多酚具有较强的清除羟基自由基的能力,是一种很好的天然抗氧剂。

关 键 词:竹叶  茶多酚  正交试验  提取  抗氧化

Study on Extraction of Tea-Polyphenols in Bamboo Leaf and its Antioxidational Effect
WANG Wenyuan,HUANG Guangwen,LONG Hongping,ZHANG Yunlan,ZHOU Jincai,ZHOU Zhenhua.Study on Extraction of Tea-Polyphenols in Bamboo Leaf and its Antioxidational Effect[J].Flavour Fragrance Cosmetics,2011(2):24-28.
Authors:WANG Wenyuan  HUANG Guangwen  LONG Hongping  ZHANG Yunlan  ZHOU Jincai  ZHOU Zhenhua
Affiliation:WANG Wenyuan1HUANG Guangwen2LONG Hongping3ZHANG Yunlan1ZHOU Jincai1ZHOU Zhenhua1(1.Hunan Yongzhou Vocational Technical College,Yongzhou 425000,China,2.Renewable Resources Institute of Hunan University of Science and Engineering,Key Lab.on Renewable Resources,Yongzhou 425007,3.School of Pharmacy,Central South University,Changsha 410013,China)
Abstract:To explore optimal process conditions of extracting tea-polyphenols from bamboo leaf by ultrasonic technology and measure its antioxidational effects, take the tea-polyphenols yield as index, based on the single factor and orthogonal tests,determine the optimum conditions of extracting tea-potyphenols from bamboo leaf by ultrasonic. The optimum conditions were ultrasonic power 70 W, extracting temperature 75℃, extracting time 40 min, ratio of solid to liquid 1/20(g/ml.), ethanol concentration 75%. Under these conditions,the extraction yield of tea-polyphenols was 0. 624 3%. The antioxidative activities, including scavenging activity of hydroxyl radicals and 1,1-diphenyl-2-picrylhydrazyl (DPPH)radicals were studied. The results indicated that the tea-polyphenols from bamboo leaf exhibited high free radical scavenging activity and it is a good natural antioxidant.
Keywords:bamboo leaf tea-polyphenols orthogonal test extraction antioxidation  
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