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蛋白质水解度测定方法综述
引用本文:徐英操,刘春红.蛋白质水解度测定方法综述[J].食品研究与开发,2007,28(7):173-176.
作者姓名:徐英操  刘春红
作者单位:东北农业大学理学院应用化学系,黑龙江,哈尔滨,150030
摘    要:对目前国内外常用的蛋白质水解度测定方法进行了综述,其中pH—state方法是通过滴定水解过程中释放的质子测定DH;OPA、TNBS及国内常用的水合茚三酮和甲醛等测定方法是利用游离氨基的反应测定DH。

关 键 词:蛋白质  水解度  游离氨基  测定
修稿时间:2006-12-04

INTRODUCTION OF METHOD FOR DETERMINATION OF DEGREE OF HYDROLYSIS OF PROTEIN HYDROLYSATES
XU Ying-cao,LIU Chun-hong.INTRODUCTION OF METHOD FOR DETERMINATION OF DEGREE OF HYDROLYSIS OF PROTEIN HYDROLYSATES[J].Food Research and Developent,2007,28(7):173-176.
Authors:XU Ying-cao  LIU Chun-hong
Affiliation:Department of Chemistry ,College of Science, Northeast Agriculture University,Harbin 150030,Heilongjiang, China
Abstract:This paper is a summary based on the current domestic and foreign used methods for measuring the degree of hydrolysis of protein hydrolysates, which pH-state method is used to determine DH (degree of hydrolysis) by protons release of the hydrolysis process in titration; OPA, TNBS and domestic commonly used, such as ninhydrin and formaldehyde are measured by free-amido's response.
Keywords:protein  degree of hydrolysis  free amidogen  determination
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