异烟肼对斑马鱼胚胎发育量-毒关系的研究

研究了一线抗结核药物异烟肼对斑马鱼发育的量-毒关系。用不同浓度的异烟肼分别处理发育至2 hpf(hour post fertilization)的斑马鱼胚胎，观察胚胎和幼鱼的死亡率、孵化率、畸形率、体长及形态学变化。急性毒性实验结果表明异烟肼对斑马鱼有明显的发育毒性，能导致斑马鱼发育迟缓和畸变。与对照组相比，低剂量（小于6 mmol/L）异烟肼对斑马鱼无发育毒性，高剂量（≥32 mmol/L）异烟肼显著抑制胚胎的孵化率，并有明显的胚胎致死效应和致畸效应。斑马鱼死亡率和畸形率均随给药剂量的增加和给药时间的延长而升高。本实验条件下，异烟肼的最大无毒性效应浓度为2 mmol/L。

Dose-dependent developmental toxicity of isoniazid to zebrafish embryo

We address dose dependent developmental toxicity of isoniazid (INH) to zebrafish. We respectively process 2hpf zebrafish embryo with different concentrations of INH, and observe the variations of the mortality of zebrafish larva and embryo, hatching rate, malformation rate, body length and morphology. Acute toxicity experiment shows that INH has significant developmental toxicity to zebrafish, and can cause developmental delay and malfunction of zebrafish. Compared with the control group, lower doses (<6 mmol/L) of INH have no developmental toxicity to zebrafish, while higher doses (≥32 mmol/L) of INH significantly inhibit hatching rate of embryos, and have obvious embryonic death and teratogenic effects. Mortality and malformation rates of zebrafish both increase with the increase of drug delivery dosage and time extension. For this experiment, the largest concentration of INH non toxic effect is 2 mmol/L.