Kinetic studies on strawberry anthocyanin hydrolysis by a thermostable anthocyanin-β-glycosidase from Aspergillus niger |
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Authors: | Hans Blom Magny S Thomassen |
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Affiliation: | Norwegian Food Research Institute, PO Box 50, N-1432 Ås-NLH, Norway |
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Abstract: | Several kinetic characteristics of a thermostable anthocyanin-β-glycosidase from Aspergillus niger have been evaluated. With strawberry anthocyanins as substrate, at pH optimum (4·0) and t = 30°C, Km was found to be and Vmax, 1·16 ± 0·06 μmol min?1mg?1 protein. Temperature optimum was observed at about 68°C. The apparent energy of activation was calculated to be 11 ± 1 kcal/mol. The inhibitory effect of different sugars and sugar derivatives was examined. Glucono-deltalactone (), gluconic acid () and glucose () appeared to be competitive inhibitors of this enzyme. |
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