糙叶败酱药材中异白花败酱醇的定性定量分析

目的:建立糙叶败酱药材中异白花败酱醇的定性和定量分析方法。方法:采用薄层色谱(TLC)法定性鉴别糙叶败酱药材中的异白花败酱醇;采用反相高效液相色谱法测定其含量,色谱柱为Agilent Eclipse plus C18(250mm×4.6mm,5μm),流动相为乙腈-磷酸水溶液(pH2.5)=7:93,流速为1.0mL.min-1,检测波长为210nm,柱温为30℃。结果:糙叶败酱药材中异白花败酱醇的TLC鉴别特征明显,专属性好;异白花败酱醇的进样浓度在0.2～1.2mg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9997),平均加样回收率为98.8%,RSD=2.84%(n=9)。结论:所建分析方法准确、可靠,可用于糙叶败酱的质量控制。

Qualitative and Quantitative Analysis of Isovillosol in Patrinia scabra

OBJECTIVE:To establish the method for qualitative and quantitative analysis of isovillosol in Patrinia scabra.METHODS:Isovillsol in Patrinia scabra was identified by TLC.The content of isovillosol was determined by RP-HPLC.The determination was performed on Agilent Eclipse plus C18(250 mm×4.6 mm,5 μm)column with mobile phase consisted of acetonitrile-phosphoric acid solution(pH=2.5,7:93) at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 210 nm and column temperature was 30 ℃.RESULTS:Isovillosol has an obvious character in the result of TLC.There was a good linear relationship in the range of 0.2～1.2 mg·mL-1(r=0.999 7).The average recoveries were 98.8%(RSD=2.84%,n=9).CONCLUSION:The established method is accurate and reliable.It is suitable for the quality control of P.scabra.