| cAMP对胰岛素刺激的NIH3T3细胞丝裂原激活蛋白激酶活性的抑制作用 |
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| 引用本文： | 何艳,叶庆林,林德馨,高永琳.cAMP对胰岛素刺激的NIH3T3细胞丝裂原激活蛋白激酶活性的抑制作用[J].福建医科大学学报,2003,37(2):144-146. |
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| 作者姓名： | 何艳 叶庆林 林德馨 高永琳 |
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| 作者单位： | 福建医科大学生物化学与分子生物学系,福州,350004 |
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| 基金项目： | 福建省教育厅科研基金资助项目 ( JA99183 ) |
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| 摘 要： | 目的 探讨cAMP-PKA信号途径对胰岛素激活的Ras-丝裂原激活蛋白激酶(MAPK)信号途径的影响。方法 以3-异丁基-1-甲基黄嘌呤(IBMX)提高细胞内环腺苷酸(cAMP)浓度，藉四唑蓝比色法(MTT法)和蛋白免疫印迹试验分别观察cAMP对胰岛素刺激的小鼠成纤维细胞(NIH3T3细胞)的生长增殖和胞内丝裂原激活蛋白激酶(MAPK)活性的影响。结果 IBMX(0．5mmol/L)抑制静息的和胰岛素(10mU／mL)刺激的NIH3T3细胞的增殖，作用呈时间依赖性。在胰岛素(10mU／mL)刺激的NIH3T3细胞，MAPK出现酪氨酸磷酸化；而以IBMX(0．5mmol/L)预处理细胞30min后，再以胰岛素作用10min，胰岛素刺激的MAPK的酪氨酸磷酸化受抑制。结论：cAMP可通过抑制MAPK磷酸化活化对NIH3T3细胞增殖起抑制作用。
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| 关 键 词： | 信号传导 胰岛素 环AMP 有丝分裂素激活蛋白激酶类 |
| 文章编号： | 1672-4194(2003)02-0144-03 |
| 修稿时间： | 2002年12月16 |
Inhibition of cAMP on Mitogen-activated Protein Kinases Activity of NIH3T3 Cells Stimulated by Insulin |
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| HE Yan,YE Qing\|lin,LIN De\|xin,GAO Yong\|lin Division of Biochemistry and Molecular Biology,Fujian Medical University,Fuzhou ,China.Inhibition of cAMP on Mitogen-activated Protein Kinases Activity of NIH3T3 Cells Stimulated by Insulin[J].Journal of Fujian Medical University,2003,37(2):144-146. |
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| Authors: | HE Yan YE Qing\|lin LIN De\|xin GAO Yong\|lin Division of Biochemistry and Molecular Biology Fujian Medical University Fuzhou China |
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| Affiliation: | HE Yan,YE Qing\|lin,LIN De\|xin,GAO Yong\|lin Division of Biochemistry and Molecular Biology,Fujian Medical University,Fuzhou 350004,China |
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| Abstract: | Objective To study the effect of cAMP\|PKA signal transduction pathway on Ras\|\{mitogen\}\|activated protein kinases(MAPK) signal pathway stimulated by insulin in NIH3T3 cell. \{Methods\} The effect of 3\|isobutyl\|1\|methylxenthine(IBMX) which increases the intracellular cAMP on the growth and activity of MAPK of NIH3T3 cells stimulated by insulin were investigated with methylthiazolyl tetrazolium(MTT) assay and Western\|blot respectively. Results IBMX(0 5 mmol/L) inhibited the proliferation of NIH3T3 cells with or without 10 mU/mL insulin. Tyrosine phosphorylation of MAPK in NIH3T3 cells stimulated with insulin was decreased when it was pretreated with IBMX for \{30 min\}. \{Conclusion\} cAMP may inhibit the growth of NIH3T3 cells by inhibiting the Ras\|MAPK signal pathway. |
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| Keywords: | signal tranduction insulin cAMP mitogen\|activated protein kinases |
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