| 氢质子磁共振波谱活体检测结肠癌耐药性及其分子标志物的探索 |
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| 引用本文： | 谢琦,陈惠娴,杜磊,廖炎辉,谭智霖,杨逸铭,吴敏仪,潘奇锋,吴锦斌,张鼎旋.氢质子磁共振波谱活体检测结肠癌耐药性及其分子标志物的探索[J].磁共振成像,2019,10(6):435-439. |
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| 作者姓名： | 谢琦 陈惠娴 杜磊 廖炎辉 谭智霖 杨逸铭 吴敏仪 潘奇锋 吴锦斌 张鼎旋 |
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| 作者单位： | 广州医科大学附属广州市第一人民医院南沙医院影像科,广州 510180;广州市第一人民医院/华南理工大学附属第二医院南沙医院影像科,广州510180;广州医科大学附属广州市第一人民医院南沙医院影像科,广州,510180;广州市第一人民医院/华南理工大学附属第二医院南沙医院影像科,广州,510180;广东省中医院影像科,广州,510030;番禺中心医院影像科,广州,511400 |
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| 摘 要： | 目的探索氢质子磁共振波谱(1H-magnetic resonance spectroscopy,1H-MRS)活体评价人类结肠癌耐药性及可能分子标志物。材料与方法 8只耐药与8只不耐药人类结肠癌SW480荷瘤裸鼠于肿瘤最大径大于1.5 cm后,分别行1H-MRS检查后,过度麻醉处死荷瘤鼠,切下肿瘤,检测肿瘤的坏死(HE染色)及P-gp、MRP1、PKC、γ-GCSh、γ-GCSl、GSHS、GST蛋白表达(Western blot)。结果耐药组人类结肠癌SW480裸鼠模型Cho峰、Lac峰、Glx1峰、Lip及Ins峰/Cr峰面积较不耐药组人类结肠癌SW480/5-FU裸鼠模型增加,差异有统计学意义(P<0.05)。在HE切片上,组耐药组与不耐药组均见少许坏死,两组组织坏死面积差别无显著性,不耐药组组织间隙较耐药组增大。耐药组PKC、P-gp、MRP1、γ-GCSh、γ-GCSl、GSHS、GST蛋白表达显著高于不耐药组(P<0.05)。Cho峰、Lac峰、Glx1峰、Lip及Ins峰/Cr峰面积与肿瘤耐药相关蛋白表达有明显相关性。结论 Cho峰、Lac峰、Glx1峰及Ins峰/Cr峰面积比有可能作为活体监测结肠癌SW480耐药性的分子标志物。
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| 关 键 词： | 磁共振波谱 磁共振成像 结肠肿瘤 移植瘤模型鼠 多药耐药 动物实验 |
Detection of the drug resistance of human colon cancer in mice with proton magnetic resonance spectroscopy |
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| XIE Qi,CHEN Huixian,DU Lei,LIAO Yanhui,TAN Zhilin,YANG Yiming,WU Minyi,PAN Qifeng,WU Jinbin,ZHANG Dingxuan.Detection of the drug resistance of human colon cancer in mice with proton magnetic resonance spectroscopy[J].Chinese Journal of Magnetic Resonance Imaging,2019,10(6):435-439. |
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| Authors: | XIE Qi CHEN Huixian DU Lei LIAO Yanhui TAN Zhilin YANG Yiming WU Minyi PAN Qifeng WU Jinbin ZHANG Dingxuan |
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| Affiliation: | (Department of Imaging,Nansha Hospital,Guangzhou First People's Hospital,Guangzhou Medical University,Guangzhou 510180,China;Department of Imaging,Nansha Hospital,Guangzhou First People's Hospital,the Second Affiliated Hospital of South China University of Technology,Guangzhou 510180,China;Guangdong Province Traditional Chinese Medical Hospital,Guangzhou 510030,China;Guangzhou Panyu Central Hospital,Guangzhou 511400,China) |
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| Abstract: | XIE Qi;CHEN Huixian;DU Lei;LIAO Yanhui;TAN Zhilin;YANG Yiming;WU Minyi;PAN Qifeng;WU Jinbin;ZHANG Dingxuan(Department of Imaging,Nansha Hospital,Guangzhou First People's Hospital,Guangzhou Medical University,Guangzhou 510180,China;Department of Imaging,Nansha Hospital,Guangzhou First People's Hospital,the Second Affiliated Hospital of South China University of Technology,Guangzhou 510180,China;Guangdong Province Traditional Chinese Medical Hospital,Guangzhou 510030,China;Guangzhou Panyu Central Hospital,Guangzhou 511400,China) |
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| Keywords: | magnetic resonance spectroscopy magnetic resonance imaging colonicneoplasms xenograft model in nude mice multiple drug resistance animal experimentation |
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