| 高效液相色谱法检测乳品中3种甜味剂 |
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| 引用本文： | 赵贞,万鹏,刘春霞,岳虹,李素琴,李翠枝.高效液相色谱法检测乳品中3种甜味剂[J].中国酿造,2014(1):147-149. |
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| 作者姓名： | 赵贞 万鹏 刘春霞 岳虹 李素琴 李翠枝 |
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| 作者单位： | 内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特010010 |
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| 摘 要： | 建立了乳制品中安赛蜜、阿斯巴甜和纽甜的高效液相色谱分析方法。样品用沉淀剂沉淀过滤后,以乙腈-磷酸二氢钠溶液为流动相,C18反相色谱柱分离,紫外检测器210nm检测。结果表明:3种甜味剂可在25min之内实现完全分离。安赛蜜和阿斯巴甜在1~100mg/kg,纽甜在0.5~100mg/kg范围内呈良好的线性关系,回收率为84.3%~102.6%。该方法用于乳制品中3种甜味剂的测定,灵敏度高,定量准确,节省时间和成本。
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| 关 键 词： | 液相色谱法 安赛蜜 阿斯巴甜 纽甜 乳制品 |
Detection of 3 kinds of sweetener in dairy products by HPLC |
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| ZHAO Zhen,WAN Peng,LIU Chunxia,YUE Hong,LI Suqin,LI Cuizhi.Detection of 3 kinds of sweetener in dairy products by HPLC[J].China Brewing,2014(1):147-149. |
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| Authors: | ZHAO Zhen WAN Peng LIU Chunxia YUE Hong LI Suqin LI Cuizhi |
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| Affiliation: | (Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot 010010, China) |
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| Abstract: | A method for detecting acesulfame-k, aspartame and neotame in dairy products by HPLC was described. The samples were precipitated and filtered, then separated on a C18 column with the mobile phase （acetonitrile-sodium dihydrogen phosphate solution）, detected with UV detector at 210nm. The result showed that three kinds of sweeteners were completely separated within 25min, when the acesulfame-k and aspartame was in the range of 1-100mg/kg, neotame was in the range of 0.5-100mg/kg, they both showed good linearity relationship and the recovery rate was 84.3%-102.6%. This method was sensitive, accurate, costless and time saving for detecting 3 kinds of sweetener in dairy products. |
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| Keywords: | HPLC acesulfame-k aspartame neotame dairy products |
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