脂多糖和甲双吡丙酮诱发形成豚鼠运动性哮喘模型

目的建立一种接近临床特点的运动性哮喘动物模型。方法豚鼠２７只分４组。实验组（Ａ组）用脂多糖（１ｍｇ／ｋｇ）和甲双吡丙酮（５０ｍｇ／ｋｇ）腹腔注射，４天后测定气道阻力和动态肺顺应性基础值，８小时后进行运动试验并复测上述指标。对照组有３组（Ｂ、Ｃ、Ｄ组）；分别为腹腔注射脂多糖和甲双吡丙酮不运动组、腹腔注射生理盐水运动组和腹腔注射生理盐水不运动组。结果实验组豚鼠在运动后肺阻力增高、动态肺顺应性降低，而３个对照组上述指标变化均无统计学意义。结论脂多糖和甲双吡丙酮腹腔注射可诱发形成豚鼠运动性哮喘模型

A model for exercise induced asthma in guinea pigs with lipopolysaccharide and metyrapone

Objective To investigate the relationship between airway inflammation and exercise induced asthma (EIA), an experimental model for EIA in guinea pigs was developed. Method 27 guinea pigs was devided into 4 groups. A)LPS MET exercise group ( n =7) had been pretreated with lipopolysaccharide (LPS 1 mg/kg, ip) and metyrapone (MET 50 mg/kg,ip) on the first day. Then lung resistance (RL) and dynamic compliance (Cdyn) were measured before and after exercise on the fourth day. B)LPS MET non exercise group ( n =6) that pretreated with LPS and MET had nonexercise on the 4th day. C)NS exercise group ( n =7) pretreated with normal saline (NS 1 mg/kg, ip) on the 1st day. RL and Cdyn were measured before and after exercise on the 4th day. D)NS nonexercise group ( n =7) that pretreated with NS had no exercise on the 4th day.Result In LPS MET exercise group RL increased and Cdyn reduced significantly after exercise. In another 3 control groups there were no such changes either in RL or in Cdyn. Conclusion These observations suggest that the treatment with LPS and MET could make a model for exercise induced asthma.