熟地和制首乌多糖对贫血小鼠骨髓有核细胞数和细胞周期的影响

目的:观察熟地多糖(RPS)和制首乌多糖(PPS)对骨髓抑制贫血小鼠外周血象、骨髓有核细胞(BMC)数和细胞周期的影响。方法:用60Co辐射和腹腔注射环磷酰胺(Cy)及氯霉素(Ch),建立骨髓抑制贫血小鼠模型,分别提取熟地和制首乌多糖,腹腔注射RPS(20mg/kg)和PPS(25mg/kg),观察对外周血象、BMC数的影响,并用流式细胞仪分析细胞周期。结果:RPS和PPS均能一定程度促进外周血象的恢复,明显提高骨髓抑制贫血小鼠BMC数量(P<0.01);流式细胞术分析表明,G0/G1期细胞比例明显减少(RPS:P<0.01,PPS:P<0.05),S期细胞比例明显增加(RPS:P<0.01,PPS:P<0.01)。结论:RPS、PPS能促进骨髓抑制贫血小鼠骨髓抑制的恢复,促进骨髓有核细胞进入增值周期。

Effct of Rehmannia glutinosa and Polignum. Multiflorum Thunb Polysaccharide on number and cell cycle of bone marrow cells in anemic mice

Objective:To research the effect of Rehmannia glutinosa and Polignum. multiflorum Thunb polysaccharide on hemogram,the number and cell cycle of bone marrow nucleated cellsBMC in anemic mice.Methods:Anemic mice model resulted from myelosuppression by irradiation and cytotoxic chemotherapeutic compounds.Extract the polysaccharide from Rehmannia glutinosa and Polignum.multiflorum Thunb.Anemic mice were treated with RPS20mgkg and PPS25mgkg by celiac injection.Count the numbers of peripheral blood cells and bone marrow nucleated cells;analyze the cell cycle by Flow Cyto MetryFCM of bone marrow nucleated cells in anemic and treated mice.Results:RPS and PPS can increase the numbers of peripheral blood cells to some degree and the number of BMC markedlyP<0.01 in anemic mice;RPS and PPS also can decrease the number of BMC in G0G1 phaseRPS:P<0.01,PPS:P<0.05 and increase the number in S phaseRPS:P<0.01,PPS:P<0.05 evidently.Conclusions:RPS and PPS can promote the recovery of myelosuppression and promote the bone marrow nucleated cells to enter the cell cycle.