| 黑斑蛙3个Sox基因HMG-box区的序列分析 |
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| 引用本文： | 刘先英,季代丽,李洁,聂刘旺.黑斑蛙3个Sox基因HMG-box区的序列分析[J].生物学杂志,2005,22(5):27-30. |
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| 作者姓名： | 刘先英 季代丽 李洁 聂刘旺 |
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| 作者单位： | 安徽师范大学生命科学学院,芜湖,241000 |
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| 基金项目： | 安徽省教育厅自然科学基金重点项目（2002kj128）安徽省自然科学基金（NO.01043202） |
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| 摘 要： | 参照人SRY基因HMG-box保守区的序列,设计一对兼并引物,扩增了黑斑蛙Sox基因并对扩增产物进行了克隆及测序.结果在雌雄个体中共筛选出三个不同的Sox基因,rnSox3,rnSox21,rnSox4其序列与人相应SOX基因的相似性分别为86%、82%、83%,与人类相应SOX基因编码氨基酸的一致性分别为94%、93%、78%.本研究为探索黑斑蛙的性别决定机制提供了分子资料.
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| 关 键 词： | Sox基因 黑斑蛙 简并PCR |
| 文章编号： | 1008-9632(2005)05-0027-04 |
| 收稿时间： | 2004-11-11 |
| 修稿时间： | 2005-04-02 |
PCR- SSCP and sequence analysis of Sox gene in the Rana Nigromaculata |
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| LIU Xian-ying,JI Dai-li,LI Jie,NIE Liu-wang.PCR- SSCP and sequence analysis of Sox gene in the Rana Nigromaculata[J].Journal of Biology,2005,22(5):27-30. |
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| Authors: | LIU Xian-ying JI Dai-li LI Jie NIE Liu-wang |
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| Affiliation: | Life Science College, Anhui Normal University, Wuhu 241000, China |
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| Abstract: | The Sox gene of Rana Nigromaculata with degenerate primers which specially amplify the conservation sequence of human HMG- box gene was amplified. Three different Sox genes were obtained from male and female body. The identify of possible sequence of obtained SOX to that of human is 82 %, 83 %, 86 %, respectively. The identify of possible amino acid sequence of obtained SOX to that of human is 94 %, 93 %, 78 %, respectively. The data for the study on Sex - determining mechanism of Rana Nigromaculata were supplied. |
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| Keywords: | Sox gene Rana Nigromaculata degenerate PCR |
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