平阳霉素对血管内皮细胞急性损伤后酶活性变化的实验研究

目的：探讨平阳霉素损伤血管内皮细胞的机制，为临床应用提供组织学依据。方法：选用成年Wistar大白鼠42只（其中6只为正常对照），经肠系膜静脉分别注入平骒霉素（24只）或鱼肝油酸钠（12只）后，分别于注药后0．5，1，2，4，8，24h，切取肝脏组织，通过酶组化染色法测定肝窦内皮细胞的5’－核苷酸酶，肝细胞的琥珀酸脱氢酶（SDH）及乳酸脱氢酶（LDH）活性，结果：注入药液后，肝窦内皮细胞的5’－核苷酸酶，肝细胞的SDH和LDH活性随时间逐渐下降，其中平阳霉素组下降较轻微，结论：平阳霉素和鱼肝油酸钠都可对血管内皮细胞产生损伤，损伤的性质一样，均为非特异性，但平阳霉素损伤程度轻微，而鱼肝油酸钠严重。

An Experimental Study of Effects of Injection of Pingyangmycin on Activities of Enzyme of Endothelial Cells

Objective:The objective of this study is to investigate the mechanism of acute trauma of endothelium after injection of Pingyangmycin. Methods:A total of 42 adult Wistar rats were divided randomly into experimental groups (24), positive control groups (12) and normal control group (6), and the rats in the experimental or the positive control groups were injected with Pingyangmycin or sodium morrhuate into the mesenteric veins respectively, and then the enzyme activity of hepatic endothelial cells and hepatocytes was examined histochemically 0.5, 1, 2, 4, 8 and 24 hours after injection.Results:After injection, the activity of 5'_nucleinase(5'_N) in hepatic endothelial cells and that of lactic dehydrogenase (LDH) and succinodehydrogenase (SDH) in hepatocytes decreased gradually, but that injected Pingyangmycin was less than that injected sodium morrhuate.Conclusion:The trauma caused by Pingyangmycin is less than that caused by sodium morrhuate, but the reaction of trauma caused by both is nonspecific.