早期静脉注射特布他林对大鼠急性肺损伤的影响

目的观察早期静脉注射特布他林注射液对大鼠急性肺损伤（ALI）的影响。方法将40只SD大鼠随机分为4组：生理盐水对照组（A组）、ALI组（B组）、ALI＋特布他林治疗组（C组）、ALI＋特布他林＋哇巴因干预组（D组），每组10只。各组大鼠分别以10％水合氯醛3．5uL·g-1腹腔麻醉。麻醉成功后，A组大鼠采用尾静脉注射生理盐水5mg·kg-1，1h内注完；B组大鼠采用尾静脉注射脂多糖（LPS）5mg·kg-1（融于1mL生理盐水中），分4次给药，1h内注完；C、D2组大鼠采用尾静脉注射LPS5mg·kg-1（融于1mL生理盐水中），分4次给药，1h内注射完后，再注射硫酸特布他林注射液5．0uL·g-1体质量，1h内注射完后。然后将大鼠头部抬高45°，光源照射颈部直视下行气管插管，插管成功后，A、B、C3组气管内滴注林格氏液1mL·kg-1，D组气管内滴注含哇巴因10-3mol·L-1的林格氏液1mL·kg-1通过建立LPS诱导的大鼠急性肺损伤模型，对各组大鼠肺组织病理学评分，观察各组大鼠的肺湿干重比（W／D）、肺组织Na＋-K＋-ATP酶的活性，并测定各组大鼠肺泡灌洗液中的细胞数、中性粒细胞计数及蛋白含量。结果A组肺组织病理学评分值、W／D值、细胞计数、中性粒细胞百分比、蛋白含量均明显低于B、C、D3组（均P〈0．05），B、D2组上述指标均明显高于C组（均P〈0．05），B组上述指标与D组比较差异无统计学意义（P〉0．05）。A组肺组织Na＋-K＋-ATP酶的活性明显高于B、C、D3组（P〈0．05），B、D2组均明显低于C组（均P〈0．05），B组与D组比较差异无统计学意义（P〉0．05）。结论早期静脉注射特布他林可通过上调肺组织Na＋K＋-ATP酶的活性来促进肺泡上皮细胞对液体重吸收，从而控制大鼠急性肺损伤的进展。

The Effect of Injection of Terbutaline Early on Rats with Acute Lung Injury

Objective To observe the effect of intravenous injection of terbutaline early on the rats with acute lung injury. Methods Forty SD rats were randomly assigned into 4 groups： NS control group（group A）, ALI group（group B）, ALI＋ terbutaline treatment group （group C） and ALI＋terbutaline＋ouabain intervention group （group D） with 10 in each group. The rats of all groups were respectively anesthetized by intraperitoneal injection of 10% chloral hydrate （3.5uL. g-1）. After being anaesthetized successfully, the rats in group A were injected with 5 mg . kg-1 NS through the tails vein within 1 hour; the rats in group B were injected with 5 mg . kg-1 LPS （which dissolved in 1 mL of NS） throgh the tails vein in 4 times within 1 hour; the rats in group C and D were injected with 5 mg . kg-1 LPS （which dissolved in 1 mL of NS） through the tails vein in 4 times within 1 hour. Then the rats were injected with 5.0 uL . g-1 terbutaline within 1 hour. Then elevated the rats head to 45 degrees,the tracheal intubation was performed under lights illuminating the rats neck,after intubated successfully, 1 mL . kg-1 Ringer＇s solution was dripped into trachea in groups A,B and C,and D with 1 mL . kg-1 Ringer＇s solution which contain 10-3 mol . L- 1 ouabain. To establish Lipopolysaccharide-induced model of acute lung injury in rats,the pathomorphologic scores of lung injury, the wet to dry weight ratio （W/ D）,and the activity of Na＋-K＋-ATPase were determined after 6 hours, and bronehoalveolar layage fluid （BALF） cells count,the percentage neutrophilicgranulocyte （PMN）,BALF concentration of protein were determined too. Results The pathomorphologic scores of lung injury,the wet to W/D,BALF cells count, the PMN,BALF concentration of protein in group A were significantly lower than that in group B,C,and D（all P〈0.05） ,the above mentioned parameters in group B and D were higher than those in group C（all P〈0.05）,but there were no significant differences between groups B and D（P〈0.05）. the activity of Na＋-K＋-ATP ase in group A were significantly lower than that in groups B,C,D（P〈0.05） ,the activity of Na＋-K＋-ATP ase in groups B and D were higher than those in group C（all P〈0.05）, but there were no significant differences between group B and D （P〉0.05）. Conclusion The intravenous injection of terbutaline early can promote alveolar epithelial fluid reabsorption in rats with ALI via the upregulation of the activity of Na＋-K＋-ATP ase in lung,and the effect could control the further development of ALI.