黄精多糖预处理对乳鼠心肌细胞缺氧/复氧损伤的保护作用

目的研究黄精多糖（Polygona-Polysaccharose,PSP）预处理对乳鼠心肌细胞缺氧/复氧（anoxia/reoxygen-ation,A/R）损伤的保护作用及与其抗氧化作用的关系。方法实验用SD新生大鼠（出生1~3 d）20只,雌雄不拘,常规方法培养心肌细胞,在培养48 h后按随机数字表法分为4组（每组重复实验6次）：正常对照组（Control组）、A/R组、缺氧预处理（anoxia preconditioning,APC）组（APC＋AR组,3次短暂缺氧复氧,再进行A/R操作）、PSP预处理组（PSP＋A/R组）。对以下指标进行检测：细胞存活率（MTT法）,培养液中乳酸脱氢酶（LDH）活性、脂质过氧化产物丙二醛（MDA）含量以及超氧化物歧化酶（SOD）活性;心肌细胞凋亡率（流式细胞仪）。结果与对照组比较,A/R组心肌细胞存活率、细胞培养液中SOD活性均明显降低（均P〈0.01）,而细胞培养液中MDA含量、LDH活性及心肌细胞凋亡率均明显增加（均P〈0.01）。与A/R组比较,APC＋A/R组与PSP＋A/R组心肌细胞存活率、细胞培养液中SOD活性均明显增加（均P〈0.01）,同时细胞培养液中MDA含量、LDH活性及心肌细胞凋亡率均明显降低（均P〈0.01）。与APC＋A/R组比较,PSP＋A/R组心肌细胞存活率、细胞培养液中MDA含量及LDH、SOD活性和心肌细胞凋亡率差异均无统计学意义（均P〉0.05）。结论 PSP预处理可减轻心肌细胞A/R损伤,具有模拟APC作用,其机制可能与PSP的抗氧化作用有关。

Study of Preconditioning with Polygona-Polysaccharose Protects Cultured Neonatal Rat Myocardial Cells against Anoxia/Reoxygenation Injury

Objective To explore the protective effects of Polygona-Polysaccharose（PSP） on neonatal rat myocardial cells with anoxia/reoxygenation（A/R） injury and the mechanisms.Methods The myocardial cells were isolated from 20 SD neonatal rats and cultured,followed by random table method divided into four groups after being cultured for 48 hours（the experiment was duplicated 6 times in each groups）,namely：normal control group,A/R group,anoxia preconditioning（APC）group（APC＋A/R group）,PSP preconditioning group（PSP＋A/R group）.Various techniques were adopted to detect the cells and cellular products：the levels of myocardial cells viability rate was assay by MTT colorimetric method;the levels of culture fluid of released lactate dehydrogenase（LDH）,superoxide dismutase（SOD）,and malondialde hyde（MAD）;the ratio of myocardial cells apoptosis was detected by Annexin-V/PI assays with flow cytometry.Results Compared with the control group,the myocardial cells viability,the levels of culture fluid of released SOD of A/R group significantly decreased（all P0.01）,whereas levels of culture fluid of released MDA,LDH,and the ratio of apoptosis significantly increased（all P0.01）.Compared with A/R group,the myocardial cells viability and the levels of intracellular SOD of APC＋A/R group and PSP＋A/R group were significantly increased（all P0.01）,while levels of culture fluid of released MDA,LDH,and the ratio of apoptosis significantly decreased（P0.01,respectively）.Compared with APC＋A/R group,the myocardial cells viability and the levels of intracellular SOD,MDA,LDH,and the ratio of apoptosis were not statistically significant（all P0.05）.Conclusion It suggests that PSP could protect myocardial cells against A/R injury,the mechanisms of which lie in that PSP could increase activities of endogenous antioxidants.