微囊化兔坐骨神经组织细胞移植对脊髓损伤大鼠比目鱼肌FasL及Caspase-3表达的影响

目的观察用微囊化兔坐骨神经组织细胞移植治疗脊髓损伤对比目鱼肌凋亡相关蛋白FasL及Caspase-3表达的影响,探讨微囊化异种组织细胞移植治疗脊髓损伤的可能机制。方法家兔8只用于制备兔坐骨神经组织细胞悬液。成年SD大鼠80只按随机数字表法分为A组（正常组8只）、B组（微囊化兔坐骨神经组织细胞移植组24只）、C组（兔坐骨神经组织细胞移植组24只）和D组（单纯损伤组24只）。B、C及D组大鼠于T10脊髓行左半横断伤后,立即于损伤处分别植入明胶海绵吸附的10μL微囊化坐骨神经组织细胞、明胶海绵吸附的10μL坐骨神经组织细胞以及空明胶海绵。分别于术后1、3、7、14 d（每个时相取6只大鼠）取损伤侧比目鱼肌肌腹,A组（每个时相取2只大鼠）取相应部位的比目鱼肌肌腹。应用HE染色观察肌细胞微观形态、免疫组织化学染色观察比目鱼肌凋亡相关蛋白FasL及Caspase-3的表达情况。结果脊髓半切损伤后B组肌纤维萎缩始终不明显,而C、D组随时间的进展肌萎缩越来越明显。脊髓损伤1 d后比目鱼肌FasL及Caspase-3阳性表达升高,至第7天达高峰,第14天维持在较高水平;B组FasL及Caspase-3的表达明显低于同时间点的C组及D组（P〈0.01）。结论微囊化兔坐骨神经组织细胞移植治疗脊髓损伤可抑制比目鱼肌FasL及Caspase-3的表达,并延缓肌萎缩。

Effects of Transplantation of Microencapsulated Rabbit Sciatic Nerve Cells/tissue on Expression of FasL and Caspase-3 in Soleus Muscle after Spinal Cord Injury in Rats

Objective To investigate the changes in the expression of fasL and caspase-3 in soleus muscle after transplantation of microencapsulated rabbit sciatic nerve cells/tissue treating spinal cord injury（SCI） in rats,and to discuss the possible mechanism of the rehabilitation induced by the transplantation of microencapsulated xeno-sciatic nerve cells/tissue.Methods The rabbit sciatic nerve cells/tissue suspension was prepared from 8 rabbits.A total of 80 adult Sprague Dawley rats were randomly divided into 4 groups：group A（normal control group,n=8）,group B（transplantation of microencapsulated xeno-sciatic nerve cells/tissue,n=24）,group C（transplantation of xeno-sciatic nerve cells/tissue,n=24） and group D（injury control group,n=24）.Left hemisection of spinal cord at T10 level was performed in group B,C and D,and subsequently the gelatin sponge sticking 10 μL microencapsulated nerve cells/tissue suspension,the gelatin sponge sticking 10 μL cells/tissue suspension and gelatin sponge were transplanted,respectively.In group B,C and D,soleus samples in damage sides were taken from 6 rats in each group 1,3,7 and 14 days after SCI,respectively,while corresponding samples were collected from 2 rats each time in normal control group.The microstructure of muscle cells was observed by HE staining,and the expression of FasL and Caspase-3 was detected by immunohistochemistry.Results After spinal cord hemisection,soleus muscle atrophy was not obvious in group B,but the amyotrophy became more obvious with time in group C and D.The expression of fasL and caspase-3 in the soleus increased 1 day after spinal cord injury,reached a maximum at day 7,and maintained at a high level at day 14.In addition,within the same time stage,the expression fasL and caspase-3 in group B was significantly lower than that in group C and group D（P0.01）.Conclusion The transplantation of microencapsulated rabbit sciatic nerve cells/tissue for the treatment of spinal cord injury can inhibit the expression of fasL and caspase-3 in soleus and delay muscle atrophy.