Endogenous hormone levels during direct somatic embryogenesis in Medicago falcata |
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Authors: | Aneta Ivanova Margarita Velcheva Plamen Denchev Atanas Atanassov Henri A Van Onckelen |
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Affiliation: | Inst. of Genetic Engineering. 2232 Kostinbrod. Bulgaria;De Montfort Univ., Inst, of Plant Molecular Biology, Kostinbrod-2, Bulgaria;Dept of Biology, UIA, Universiteitsplein I. B-2610 Wilrijk, Belgium |
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Abstract: | Endogenous indole-3-acetic acid, abscisic acid and cytokinins (zeatin, zeatin riboside, N-isopentenyladenine and N-isopentenyladenosine) were evaluated in initial explants (leaves) of in vitro propagated plants of alfalfa ( Medicago falcata L.) lines varying in embryogenic capacity and during the somatic embryogenesis process. Fast embryo-genic induction was correlated with high IAA and low ABA levels in the initial explants. No significant differences were observed in the cytokinin contents. Our results suggest that a certain hormone balance is necessary to allow the expression of the embryogenic potential. The consistent stages of the direct somatic embryogenesis are also characterized by changes in hormonal levels. |
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Keywords: | Abscisic acid alfalfa auxins cytokinins Medicago falcata somatic embryogenesis |
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