籼稻幼穗细胞悬浮培养物的胚胎发生和植株再生

籼稻幼穗诱导的愈伤组织,培养在MS基本成分并附加1—7 mg/l 2,4-D和5％椰子汁的液体培养基中,得到胚性小细胞团。这些细胞的特征是:形状小而圆,细胞壁薄,细胞内为稠密的胞质所充满,没有或具小的液泡,具淀粉粒。把它们转移到含1 mg/l BA的琼脂培养基中,在扫描电镜下观察到球形、心形、梨形和成熟胚,两周后萌发成具根、芽的完整植株。10—14天龄的悬浮培养物具有较高的再生植株能力。

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM SUSPENSION CULTURES OF YOUNG INFLORESCENCES OF INDICA RICE

Calli derived from young inflorescences of indicn rice were placed in MS liquid medium supplemented with 1-7 mg/1 2,4-D pnd 5 % coconut milk and developed into small embryogenic clusters which were consisted of small, round and thin-walled cells with dense cytoplasm containing starch granules, but small or no vactioles. when the clusters were transferred to MS agar medium supplemented with 1 mg/1 BA, some were found at globular-, heart-, pear-shaped and mature embyo stages by scanning electromicroscope, and the embryoids germinated into intact plantlets after two weeks. 10-to 14-day old suspension cultures had higher capacity to regenerate plantlets.