克罗诺杆菌的随机多态性扩增DNA(RAPD)基因分型研究

研究了克罗诺杆菌标准菌株和样品中分离菌株的分子特征,为克罗诺杆菌的种间鉴别和分子溯源提供一种有效手段。采用随机引物扩增多态性DNA分析对38株菌株进行基因分型。筛选出1条随机引物,每一菌株可扩增出1～7条DNA片段,片段在400-3500bp,可将8株标准菌株的种间区分开来。以相似性50%为标准,38株克罗诺杆菌按照其遗传差异可以分为8个基因型类群。所建立的RAPD技术可用于克罗诺杆菌的种间鉴别和分子溯源研究。

Application Study on RAPD in Genotyping of Cronobacter spp. Strains

To provide an effective means for species identification and epidemiological tracing for Cronobacter spp.strains by studying molecular characteristic of reference strains and strains isolated from samples,38 Cronobacter spp.strains were identified by genotyping test-Genomic DNA was extracted by methods of Randomly Amplified Polymorphic DNA analysis(RAPD).One effective primer was selected with which each strain could be amplified 1 to 7 DNA fragments of 400~2 000bp.8 reference strains were identified into separate species groups.Similar to 50% for the standard,38 strains could be classified into 8 genotyping groups by genetic differences.RAPD method in this study can be applied to species identification and epidemiological tracing for Cronobacter spp.strains.