脑出血大鼠脑组织ICAM-1的表达与脑含水量的变化及药物对其影响

目的探讨脑出血后血肿周围脑组织炎性损伤的病理发展过程。方法健康Wister大鼠72只随机分成脑出血模型组、假手术组、麝香组,分别于3h、24h、72h、7d取血肿周围脑组织检测细胞间黏附因子-1(ICAM-1)的表达及出血侧脑组织含水量的测定。结果与假手术组比较,脑出血组ICAM-1从3h起即有表达,72h达高峰,持续7d仍有表达(P<0.05)。脑含水量在24h后明显增加(P<0.01),7d时与假手术组无显著性差异。结论ICAM-1在血肿周围的高表达推测是脑出血后水肿形成和缺血性损伤的主要原因之一。减少ICAM-1的表达和脑含水量推测是复方麝香注射液的药理机制。

Relationships between expression of the intercellular adhesion molecule-1 and brain edema after intracerebral hemorrhage in rats and the effect of compound injection of muskiness on it

Objective To explore pathogenesis ofthe inflammatoryinjuryinhematoma surrounding tissue after intracerebral hemorrhage (ICH). Methods 72 adult male Wister rats were randomly divided into 3 groups: ICH group, ICH with muskiness group and sham-operated group, of which hematoma surrounding tissues were taken, at 3 h, 24 h, 72 h, 7 d after ICH, and examined in the expression of the intercellular adhesion molecule-1(ICAM-1) and brain water content. Results Compared with the sham-operated group, the expression of ICAM-1 in ICH group started to increase at 3 h, peaked at 72 h, and continued to 7 d(P<0.05). The brain water content increased prominently at 24 h, had no significant change at 7 d compared with sham-operated group(P<0.01). Conclusion The over-expression of ICAM-1 may be the leading cause to the perihematoma edema formation and ischemic injury. The compound injection of mushiness can reduce ICAM-1 expression and the brain water content.