8-溴-7-甲氧基白杨素对人卵巢癌A2780 细胞凋亡的作用及其机制

目的探讨8-溴-7-甲氧基白杨素（8-bromo-7-methoxychrysin, BrMC）诱导人卵巢癌A2780细胞凋亡的作用及其可能机制.方法采用不同浓度（2.5、5.0和10.0μmol·L^-1）的BrMC处理体外培养的A2780细胞,通过碘化丙啶（PI）染色流式细胞术（FCM）和ELISA法检测细胞凋亡的情况,Western Blot检测Bim和caspase-3蛋白的表达水平.结果不同浓度（2.5、5.0和10.0μmol·L^-1）BrMC均可促进A2780细胞的凋亡,且细胞凋亡率随BrMC浓度的升高而上升.不同浓度（5.0和10.0μmol·L^-1）BrMC作用的A2780细胞中Bim蛋白的表达均较正常对照组显著增加,而通过siRNA干扰下调Bim蛋白的表达能显著抑制BrMC诱导的A2780细胞凋亡.结论 BrMC可诱导人卵巢癌A2780细胞凋亡,促进Bim蛋白的表达可能是其作用机制之一.

Effect of BrMC on the Apoptosis in Human Ovarian Cancer A2780 Cells and its Mechanisms

ABSTRACT：Objective To investigate the effects of 8-bromo-7-methoxychrysin （BrMC） on the apoptosis in human ovarian cancer cells and its possible mechanisms. Methods Human ovarian cancer A2780 cells were cultured in vitro and treated with different concentrations （2.5, 5.0, 10.0μmol·L^-1） of BrMC and 50μmol·L^-1 chrysin. The apoptosis were evaluated by flow cytometry （FCM） after propidium iodide （PI） staining and enzyme-linked immunosorbent assay （ELISA）. The expressions of Bim and caspase-3 protein were analyzed by Western Blot. Results Different concentrations（2.5, 5.0, 10.0μmol·L^-1） of BrMC significantly increased the apoptotic rates in A2780 cells in a concentration dependent manner. Furthermore, different concen-trations （5.0, 10.0μmol·L%^-1） of BrMC efficaciously up-regulated the Bim expression. But down-regulation of Bim by siRNA significantly inhibited the apoptosis and caspase-3 cleavage in A2780 cells induced by BrMC. Conclusion BrMC could obviously induce the apoptosis in human ovarian cancer A2780 cells, and up-regulation of Bim expression may be involved in this process.