Phylogenetic clustering of 4 prevalent virulence genes in <Emphasis Type="Italic">Orientia tsutsugamushi</Emphasis> isolates from human patients |
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Authors: | Hyuk Chu Sang-Hee Park Eun-Ju Kim Kyu-Jam Hwang Soo-Kyoung Shim Sungdo Park Mi-Yeoun Park |
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Affiliation: | (1) Dipartimento di Etologia Ecologia Evoluzione, Università di Pisa, Italy; |
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Abstract: | The pathogenicity of microbes is involved in many kinds of virulence genes. The relationships between these virulence genes
and strains are not clear in Orientia tsutsugamushi yet. In this study, we confirmed the presence of the virulence genes and classified into O. tsutsugamushi isolates using phylogenetic analysis of the virulence genes. We also compared the fatality rates of every isolate via an
infection experiment in BALB/c mice using the O. tsutsugamushi isolates, Deajeon03-01, Wonju03-01, and Muju03-01. Moreover, we compared the phylogenetic analysis, in basis with 56 kDa
protein sequence which determined from serotype, and virulence genes of O. tsutsugamushi. Our results showed remarkably different fatality rates between Deajeon03-01 and Muju03-01, which are both Boryong strains
of O. tsutsugamushi. Also, clustering analyses including these two isolates gave slightly different results depending on whether they were clustered
based on virulence genes or on the 56 kDa protein sequences. Consequently, we conclude that fatality rates in O. tsutsugamushi are correlated with differences in both serotypes and virulence genes. We identified some variations within the virulence
genes dnaA, virB8, tolR, and trxA among the isolates. |
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