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| 实验性肝纤维化逆转过程中基质金属蛋白酶表达的动态研究 | |
| 引用本文： | 赵志海,辛绍杰,赵景民,王松山,周光德,程云,赵平,靳雪源.实验性肝纤维化逆转过程中基质金属蛋白酶表达的动态研究[J].临床肝胆病杂志,2007,23(1):21-23. |
| 作者姓名： | 赵志海 辛绍杰 赵景民 王松山 周光德 程云 赵平 靳雪源 |
| 作者单位： | 1. 解放军军医进修学院,解放军302医院十二科,北京,100039 2. 解放军军医进修学院,解放军302医院三科,北京,100039 3. 解放军军医进修学院,解放军302医院病理科,北京,100039 4. 解放军军医进修学院,解放军302医院传染病研究所,北京,100039 |
| 基金项目： | 军队“九五”医药卫生基金（编号98M152） |
| 摘 要： | 探讨基质金属蛋白酶在实验性肝纤维化逆转过程中的表达及意义。建立大鼠实验性四氯化碳中毒性肝纤维化模型，采用核酸原位杂交和免疫组化法检测实验性肝纤维化自然逆转过程中MMP-13、MMP-2、MT—MMP2的来源细胞、时序和量的变化。MMP-13、MMP-2、MT—MMP2在间质细胞和部分肝细胞表达，MMP-13先维持在一定水平，然后逐渐减弱；MMP-2、MT—MMP2先增强后减弱。肝脏间质细胞是基质金属蛋白酶的主要来源细胞，MMP-13、MMP-2、MT—MMP2表达与肝纤维化程度相关，在肝纤维化逆转过程中起重要作用。 |
| 关 键 词： | 肝硬化 基质金属蛋白酶 免疫组织化学 原位杂交 |
| 文章编号： | 1001-5256（2007）01-0021-03 |
| 收稿时间： | 2006-01-09 |
| 修稿时间： | 2006-03-09 |
Dynamic expression of MMP-13, MMP-2, MT-MMP2 in reversal of experimental hepatic fibrosis in rats |
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| ZHAO Zhi-hai, XIN Shao-jie, ZHAO Jing-min,et al..Dynamic expression of MMP-13, MMP-2, MT-MMP2 in reversal of experimental hepatic fibrosis in rats[J].Chinese Journal of Clinical Hepatology,2007,23(1):21-23. | |
| Authors: | ZHAO Zhi-hai XIN Shao-jie ZHAO Jing-min |
| Affiliation: | Military Postgraduate Medical School, Beijing 100039, China |
| Abstract: | To discuss the dynamic expression and significance of matrix metalloproteinase(MMP)-13,MMP-2,MT-MMP2 in reversal of experimental hepatic fibrosis in rats.Experimental hepatic fibrosis rat models induced by CCl_4 were prepared.In situ hybridization and immunohistochemistry methods were respectively used to detect MMP-13,MMP-2,MT-MMP2 mRNA and the related antigens in liver.MMP-13,MMP-2,MT-MMP2 were expressed in mesenchymal cells and parts of hepatocytes.Expression of MMP-13,MMP-2,MT-MMP2 were decreased gradually in fibrosis reversal.It was suggested that mesenchymal cells were the main cellular origins of MMPs.The level of expression of MMPs was closely related to hepatic fibrosis reversal.MMPs may play importantly roles in the reversal of hepatic fibrosis. |
| Keywords: | liver cirrhosis matrix metalloproteinases immunohistochemistry in situ hybridization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
