热适应大鼠下丘脑基因差异表达的研究

目的：从基因表达角度研究热适应的分子机制，方法：制备热适应大鼠,发离热适应和常态大鼠各自的下丘脑组织，运用差异显示PCR技术筛选两种组织之间存在的差异表达基因片段，经斑点杂交鉴定后进行测序分析。结果：从热适应大鼠下丘脑组织中分离并鉴定了一条表达增高的基因片段，经测序分析确定其为长164bp的cDNA序列。结论：热适应大鼠下丘脑存在基因的表达增高，这种基因的表达水平的变化对于动物维护热适应能力可能具有重要作用。

Study on Differential Expression of Genes in Hypothalamus of Heat Acclimated Rats

Objective The gene expression was studied to investigate the molecular mechanism of heat acclimation.Methods Heat acclimation model of rats was made first,then the hypothalamus tissue was isolated from the model rats and the normal ones respectively.The genes expressed differentially between these two kinds of tissue were identified using the differential display PCR technique and confirmed by dot blot hybridization of total RNA,then were sequenced and analyzed.Results A piece of cDNA was selected for its higher expression in the hypothalamus of heat acclimated rats and was determined to be a sequence with 164bp.Conclusion The results suggest that genes were differentially expressed in heat acclimated animals compared with normal ones,which may contribute a lot to maintaining their ability of heat adaptation.