降糖消渴颗粒对2型糖尿病大鼠糖脂代谢的影响

目的： 探讨肝脾肾同治的组方降糖消渴颗粒对2型糖尿病大鼠糖脂代谢的影响。方法： SD大鼠高脂饲料喂养4周后结合腹腔注射链脲佐菌素（STZ）30 mg·kg^-1，建立2型糖尿病大鼠模型，将成模的大鼠按血糖随机分为模型组、阳性对照组（二甲双胍0.2 g·kg^-1）、降糖消渴颗粒高、中、低剂量组（按生药量计为17.6，8.8，4.4 g·kg^-1），并设正常对照组。干预4周后，观察治疗期间大鼠的一般情况，检测大鼠空腹血糖、血脂、空腹血清胰岛素、糖化血红蛋白及糖耐量、胰岛素耐量。结果： 降糖消渴颗粒可降低糖尿病模型大鼠体重（P<0.05），与模型组相比，可降低空腹血糖（P<0.05），使胰岛素敏感指数水平升高（P<0.05），糖化血红蛋白与模型组比较有一定的改善，但差异无统计学意义。而血清胰岛素水平、血脂水平未见明显变化。给药后大鼠的糖耐量得以改善（P<0.05），且呈现良好的时-效关系。胰岛素耐量实验显示降糖消渴颗粒各剂量组均具有一定的增强胰岛素敏感性的作用。结论： 立足于肝、脾、肾三脏同治的中药复方降糖消渴颗粒能改善2型糖尿病大鼠糖、脂代谢及胰岛功能，其改善2型糖尿病胰岛素抵抗可能是其防治糖尿病的机制之一。

Effect of Jiangtang Xiaoke Granule on Glucose and Lipid Metabolism in Type 2 Diabetic Rats

Objective: To investigate the effect of the Jiangtang Xiaoke granule (JTXKG) on the blood glucose and blood lipids in type 2 diabetic rats. Method: Diabetic rats were constructed by high-fat-diet for 4 weeks and combined with intraperitoneal injection of streptozocin 30 mg·kg^-1, and the model rats were randomly divided into diabetes model group (T2DM), positive control group (metformin 0.2 g·kg^-1) JTXKG groups with high, medium, and low dose (raw herbs 17.6, 8.8, 4.4 g·kg^-1), and the normal control group (NC) was also designed. After 4-week treatment, the general condition of rats was observed, and fasting blood glucose (FBG), blood lipid (BL), fasting serum insulin (INS), glycosylated hemoglobin (HbA1c) were tested, glucose tolerance and insulin tolerance were measured. Result: Compared with model group, body weight,FBG and insulin sensitive index levels of JTXKG groups were improved (P<0.05), HbA1c showed certain changes, but the difference was not statistically significant. However, the serum insulin levels and blood lipid levels did not significantly change. After treatment, the glucose tolerance presents time-related improvement in diabetic rats, the results of insulin tolerance test showed that JTXKG could enhance insulin sensitivity of diabetic rats. Conclusion: JTXKG, the treating principle of which is regulating function of liver spleen and kidney together, can improve glucose and lipid metabolism and pancreatic islet function in type 2 diabetic rats, and relieve insulin resistance that may be the possible mechanism of JTXKG preventing and controlling type 2 diabetes.