失神经骨骼肌提取液对雪旺细胞体外增殖的作用

目的：观察去神经支配骨骼肌粗提液在体外培养条件下对雪旺细胞（Schwann cells,SCs)增殖的作用，了解外周神经损伤后SCs大量增殖的机理。方法：将新生大鼠SCs培养标本分为加正常骨骼肌提取液组，加失神经支配骨骼肌提取液组和正常对照组，每组10个标本。通过活细胞计数和S－100免疫细胞化学染色证实，观察两种肌肉提取液对SCs增倍时间的影响。结果：对照组SCs增倍时间为8天，正常骨骼肌提取液组为7天，而去神经支配骨骼肌提取液组为5．5天。结论：周围神经损伤后SCs可能受失神经支配骨骼肌产生的某些物质的刺激而增殖加快。

Effects of Denervated Skeletal Muscle Extract on Proliferation of Schwann Cells in vitro

Objective:To investigate the effects of denervated skeletal muscle extract on proliferation of Schwann cells(SCs) in vitro and elucidate the mechanism of proliferation of SCs after peripheral nerve injury.Methods:The crude normal skeletal muscle extract(NME) and denervated skeletal muscle extract(DME) were prepared in advance.The cultured SCs derived from the neonate Sprague Dawley rats were divided into NME,DME and control group,10 samples in each group.NME,DME and no extract were added into the medium of NME,DME and control group respectively.And then the time of SCs double number was evaluated by living cell count and S 100 immunocytochemistry stain.Results:The time of SCs double number is 8 days in control group,7 days in NME group and 5 5 days in DME group.Conclusion:After peripheral nerve injury,the factors produced by dennervated skeletal muscles may contribute to the increase of Schwann cells proliferation.