| 轮状病毒形态发生时病毒性抗原的免疫胶体金标记定位 |
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| 引用本文： | 陈维康,夏诗茂.轮状病毒形态发生时病毒性抗原的免疫胶体金标记定位[J].病毒学报,1989,5(2):116-122. |
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| 作者姓名： | 陈维康 夏诗茂 |
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| 作者单位： | 中国预防医学科学院病毒学研究所 上海医科大学微生物教研室北京
(陈维康)，中国预防医学科学院病毒学研究所 北京
(夏诗茂，方肇寅)，中国预防医学科学院病毒学研究所 北京(洪涛) |
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| 摘 要： |
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| 关 键 词： | 轮状病毒 抗原定位 免疫技术 |
ANTIGENIC LOCALIZATION DURING ROTAVIRUS MORPHO GENESIS BY IMMUNE COLLOID GOLD TECHNIQUE |
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| Chen Weikang Xia Shimao Fang Zhaoyin Hong Tao.ANTIGENIC LOCALIZATION DURING ROTAVIRUS MORPHO GENESIS BY IMMUNE COLLOID GOLD TECHNIQUE[J].Chinese Journal of Virology,1989,5(2):116-122. |
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| Authors: | Chen Weikang Xia Shimao Fang Zhaoyin Hong Tao |
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| Abstract: | Antigenic localization by means of immune colloid gold technique to label the pre-or post-embedding cells infected with rotavirus (SA11) , demonstrated that the VP7, the main antigen in outer capsid of double shelled viron is distributed asymmetrically on the cytoplasmic side of rER membrane. Viral structural proteins are not present,on the surface of enveloped viron (85nm) within rER lumen. The perinuclear space and outer membrane of the nucleus share a common function with rER during the morphogenesis of rotavirus. The results also show that viral cores (50nm ) are formed within the matrix-like inclusion body. The inner capsid of single shelled viroas (65nm)is assembled at the peripheral region of the inclusion. The envelopment mechanism may play an important role in rotavirus immunity in terms of escaping from host defence. |
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| Keywords: | Viral antigenic localization Rotavirus morphogenesis Immune colloid gold labeling technique |
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