| Extraction DNA from Activated Sludge-Comparing with Soil Sample |
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| 引用本文： | 谢冰,奚旦立,陈季华. Extraction DNA from Activated Sludge-Comparing with Soil Sample[J]. 东华大学学报(英文版), 2003, 20(1) |
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| 作者姓名： | 谢冰 奚旦立 陈季华 |
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| 作者单位： | School of Environmental Science and Engineering，Donghua University，Shanghai，200051，School of Environmental Science and Engineering，Donghua University，Shanghai，200051，School of Environmental Science and Engineering，Donghua University，Shanghai，200051 |
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| 摘 要： | DNA directly extraction from activated sludge andsoil sample with enzyme lyses methods wasinvestigated in this paper. DNA yield from activatedsludge was 3. 0 mg/g. MLSS, and 28. 2-43. 8μg/g soilrespectively. The resulting DNA is suitable for PCR.By studied methods, higher quality and quantity of
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Extraction DNA from Activated Sludge-Comparing with Soil Sample |
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| XIE Bing,XI Dan-li,CHENG Ji-huaSchool of Environmental Science and Engineering,Donghua University,Shanghai. Extraction DNA from Activated Sludge-Comparing with Soil Sample[J]. Journal of Donghua University, 2003, 20(1) |
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| Authors: | XIE Bing XI Dan-li CHENG Ji-huaSchool of Environmental Science Engineering Donghua University Shanghai |
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| Affiliation: | School of Environmental Science and Engineering,Donghua University,Shanghai,200051 |
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| Abstract: | DNA directly extraction from activated sludge and soil sample with enzyme lyses methods was investigated in this paper. DNA yield from activated sludge was 3.0 mg/g. VLSS, and 28.2-43.8 μg/g soil respectively. The resulting DNA is suitable for PCR.By studied methods, higher quality and quantity of sludge DNA could be obtained rapidly and inexpensively from large number of samples, and the PCR product obtained from this protocol was not affected by contaminated higher concentration of heavy metals. |
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| Keywords: | DNA extraction PCR (polymerase chain reaction) activated sludge soil heavy metal. |
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