| LC—MS／MS法快速测定中成药与保健食品中非法添加20种化学成分研究 |
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| 引用本文： | 孙夏荣,李丹,文红梅,崔福春,黄亚,郭春.LC—MS／MS法快速测定中成药与保健食品中非法添加20种化学成分研究[J].中国药师,2011,14(1):22-25. |
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| 作者姓名： | 孙夏荣 李丹 文红梅 崔福春 黄亚 郭春 |
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| 作者单位： | 1. 南京市药品检验所,南京,210015
2. 南京中医药大学 |
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| 基金项目： | 南京市2008公共安全类科技发展计划项目 |
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| 摘 要： | 目的：建立中成药与保健食品中非法添加20种降糖类化学成分的快速测定方法。方法：采用液相色谱．串联质谱法（LC—MS／MS），以Sunfire^TM C18（100mm×2．1mm，5．0μm）为分析柱，流动相采用乙腈．1％甲酸（75：25），电喷雾电离（ESI）、多反应监测（MRM）扫描方式，对中成药、保健食品中非法添加的20种化学成分进行快速定性定量分析。结果：建立了快速分离检测中成药、保健食品中20种非法添加成分的测定方法，检测限低于20ng。结论：该方法专属性强、灵敏度高，适用于降糖类中成药、保健品中非法添加成分的定性定量分析。
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| 关 键 词： | 液质联用色谱 多反应监测 化学成分 快速测定 |
Rapid Determination of 20 Illegally Added Chemical Components in Chinese Traditional Patent Medicine and Health Food by LC-MS/MS |
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| Sun Xiarong,Li Dan,Wen Hongmei,Cui Fuchun,Huang Ya,Guo Chun.Rapid Determination of 20 Illegally Added Chemical Components in Chinese Traditional Patent Medicine and Health Food by LC-MS/MS[J].China Pharmacist,2011,14(1):22-25. |
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| Authors: | Sun Xiarong Li Dan Wen Hongmei Cui Fuchun Huang Ya Guo Chun |
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| Affiliation: | Sun Xiarong~1,Li Dan~1,Wen Hongmei~2,Cui Fuchun~1,Huang Ya~1,Guo Chun~1(1.Nanjing Institute for Drug Control,Nanjing 210015,China,2.Nanjing University of Traditional Chinese Medicine) |
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| Abstract: | Objective： To establish a liquid chromatography-tandem mass chromatography （LC-MS/MS） method for the simultaneous screening 20 anti-diabetic chemical components in health care food and Chinese traditional patent medicine. Method： This method involved liquid chromatography-tandem mass spectrometry,with SunfireTM Cms cotumn （ 100 mm ×2. 1 mm,5.0 μm）. The mobile phase consisted of 0. 1% formic and acetonitrile （25：75）. The target compounds were analyzed with LC-MS/MS-ESI. Multiple-reaction monitoring （MRM） was used to screen 20 chemical components in health care food and Chinese traditional patent medicine. Result： A fast and sensitive liquid chromatography mass spectrometric （LC/MS） method for the simultaneous screening of 20 chemical components was described. The detection lowest limit of these substances was below 20 ng. Conclusion： The method is sufficiently selective and sensitive to detect chemical components in health care food and Chinese traditional patent medicine. |
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| Keywords: | LC-MS/MS Multiple-reaction monitoring(MRM) Chemical components Rapid determination |
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